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Extraction of Protein from Four Different Seaweeds Using Three Different Physical Pre-Treatment Strategies.
O' Connor, Jack Meaney, Steve Williams, Gwilym A Hayes, Maria
O' Connor, Jack
Meaney, Steve
Williams, Gwilym A
Hayes, Maria
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Date
2020-04-24
Date Submitted
Keywords
autoclave
high pressure processing
PROTEINS
seaweeds
solubility
total and free amino acids
traditional protein extraction
high pressure processing
PROTEINS
seaweeds
solubility
total and free amino acids
traditional protein extraction
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molecules-25-02005-v2.pdf
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Abstract
Seaweeds are a rich source of protein and can contain up to 47% on the dry weight basis. It is challenging to extract proteins from the raw biomass of seaweed due to resilient cell-wall complexes. Four species of macroalgae were used in this study-two brown, Fucus vesiculosus and Alaria esculenta, and two red, Palmaria palmata and Chondrus crispus. Three treatments were applied individually to the macroalgal species: (I) high-pressure processing (HPP); (II) laboratory autoclave processing and (III) a classical sonication and salting out method. The protein, ash and lipid contents of the resulting extracts were estimated. Yields of protein recovered ranged from 3.2% for Fucus vesiculosus pre-treated with high pressure processing to 28.9% protein recovered for Chondrus crispus treated with the classical method. The yields of protein recovered using the classical, HPP and autoclave pre-treatments applied to Fucus vesiculosus were 35.1, 23.7% and 24.3%, respectively; yields from Alaria esculenta were 18.2%, 15.0% and 17.1% respectively; yields from Palmaria palmata were 12.5%, 14.9% and 21.5% respectively, and finally, yields from Chondrus crispus were 35.2%, 16.1% and 21.9%, respectively. These results demonstrate that while macroalgal proteins may be extracted using either physical or enzymatic methods, the specific extraction procedure should be tailored to individual species.
Language
en
Citation
ISSN
eISSN
1420-3049
ISBN
DOI
10.3390/molecules25082005
PMID
32344706