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Endometrial aspiration biopsy: a non-invasive method of obtaining functional lymphoid progenitor cells and mature natural killer cells.
McMenamin, Moya Lysakova-Devine, Tatyana Wingfield, Mary O'Herlihy, Colm O'Farrelly, Cliona
McMenamin, Moya
Lysakova-Devine, Tatyana
Wingfield, Mary
O'Herlihy, Colm
O'Farrelly, Cliona
Advisors
Editors
Other Contributors
Date
2012-09
Date Submitted
Keywords
Other Subjects
Subject Mesh
Antigens, CD34
Antigens, CD45
Antigens, CD56
Biopsy
Dilatation and Curettage
Endometrium
Female
Flow Cytometry
Humans
Killer Cells, Natural
Laparoscopy
Lymphoid Progenitor Cells
Prospective Studies
Stem Cells
Uterus
Antigens, CD45
Antigens, CD56
Biopsy
Dilatation and Curettage
Endometrium
Female
Flow Cytometry
Humans
Killer Cells, Natural
Laparoscopy
Lymphoid Progenitor Cells
Prospective Studies
Stem Cells
Uterus
Planned Date
Start Date
Collaborators
Principal Investigators
Alternative Titles
Publisher
Abstract
The aim of this study was to compare the efficacy of endometrial aspiration biopsy (EAB) with the more traditional dilatation and curettage (D&C) for the procurement of lymphoid progenitor cells and uterine natural killer (NK) populations in endometrial tissue. This prospective observational study conducted in a tertiary referral university hospital examined endometrium obtained from 32 women admitted for laparoscopic gynaecological procedures. Each participant had endometrium sampled using both EAB and D&C. Both methods were assessed as a source of uterine NK and lymphoid progenitor cells. Similar proportions of mature CD45+CD56+ NK cells (range 25.4-36.2%) and CD45+CD34+ lymphoid progenitors (range 1.2-2.0%) were found in tissue obtained using both EAB and D&C. These cells were adequate for flow cytometric analysis, magnetic bead separation and culture. Colony formation by the CD34+ population demonstrated maturational potential. Tissues obtained via endometrial biopsy and D&C are equivalent, by analysis of uterine NK and lymphoid progenitor cells. The aim of this study was to compare two methods of endometrial sampling - endometrial aspiration biopsy and traditional dilatation and curettage - for the procurement of haematopoietic stem cells and uterine natural killer (NK) populations in endometrial tissue. Thirty-two women who had gynaecological procedures in a tertiary referral hospital participated in this study and had endometrial tissue collected via both methods. Similar populations of mature NK cells and haematopoietic stem cells were found in tissue obtained using both endometrial aspiration biopsy and dilatation and curettage. Tissue obtained via endometrial aspiration biopsy was adequate for the culture and growth of haematopoietic stem cells. We conclude that tissue obtained via endometrial biopsy and dilatation and curettage is equivalent, by analysis of uterine NK and haematopoietic stem cells using flow cytometry. This has implications for further more extensive study of endometrial haematopoietic stem cell populations, as these samples can be collected in a clinical setting without a requirement for general anaesthesia.
Language
en
ISSN
1472-6491
eISSN
ISBN
DOI
10.1016/j.rbmo.2012.05.001
PMID
22796232