Serendipitous identification of natural intergenotypic recombinants of hepatitis C in Ireland.
Fanning, Liam J
AffiliationMolecular Virology Diagnostic & Research Laboratory, Department of Medicine, Clinical Sciences Building, Cork University Hospital, Cork, Ireland. email@example.com
MeSHAmino Acid Sequence
Molecular Sequence Data
Nucleic Acid Hybridization
Sequence Analysis, Protein
Viral Nonstructural Proteins
MetadataShow full item record
CitationSerendipitous identification of natural intergenotypic recombinants of hepatitis C in Ireland. 2006, 3:95 Virol. J.
AbstractBACKGROUND: Recombination between hepatitis C single stranded RNA viruses is a rare event. Natural viable intragenotypic and intergenotypic recombinants between 1b-1a, 1a-1c and 2k-1b, 2i-6p, respectively, have been reported. Diagnostically recombinants represent an intriguing challenge. Hepatitis C genotype is defined by interrogation of the sequence composition of the 5' untranslated region [5'UTR]. Occasionally, ambiguous specimens require further investigation of the genome, usually by interrogation of the NS5B region. The original purpose of this study was to confirm the existence of a suspected mixed genotype infection of genotypes 2 and 4 by clonal analysis at the NS5B region of the genome in two specimens from two separate individuals. This initial identification of genotype was based on analysis of the 5'UTR of the genome by reverse line probe hybridisation [RLPH]. RESULTS: The original diagnosis of a mixed genotype infection was not confirmed by clonal analysis of the NS5B region of the genome. The phylogenetic analysis indicated that both specimens were natural intergenotypic recombinant forms of HCV. The recombination was between genotypes 2k and 1b for both specimens. The recombination break point was identified as occurring within the NS2 region of the genome. CONCLUSION: The viral recombinants identified here resemble the recombinant form originally identified in Russia. The RLPH pattern observed in this study may be a signature indicative of this particular type of intergenotype recombinant of hepatitis C meriting clonal analysis of NS2.
- A natural intergenotypic recombinant of hepatitis C virus identified in St. Petersburg.
- Authors: Kalinina O, Norder H, Mukomolov S, Magnius LO
- Issue date: 2002 Apr
- Identification of hepatitis C virus 2k/1b intergenotypic recombinants in Georgia.
- Authors: Zakalashvili M, Zarkua J, Weizenegger M, Bartel J, Raabe M, Zangurashvili L, Kankia N, Jashiashvili N, Lomidze M, Telia T, Kerashvili V, Zhamutashvili M, Abramishvili N, Hedskog C, Chodavarapu K, Brainard DM, McHutchison JG, Mo H, Svarovskaia E, Gish RG, Rtskhiladze I, Metreveli D
- Issue date: 2018 Mar
- Use of sequence analysis of the NS5B region for routine genotyping of hepatitis C virus with reference to C/E1 and 5' untranslated region sequences.
- Authors: Murphy DG, Willems B, Deschênes M, Hilzenrat N, Mousseau R, Sabbah S
- Issue date: 2007 Apr
- Natural intragenotypic and intergenotypic HCV recombinants are rare in southwest China even among patients with multiple exposures.
- Authors: Zhou Y, Wang X, Hong G, Tan Z, Zhu Y, Lan L, Mao Q
- Issue date: 2010 Dec
- Novel nucleotide and amino acid covariation between the 5'UTR and the NS2/NS3 proteins of hepatitis C virus: bioinformatic and functional analyses.
- Authors: Sun HY, Ou NY, Wang SW, Liu WC, Cheng TF, Shr SJ, Sun KT, Chang TT, Young KC
- Issue date: 2011