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Carlow virus, a 2002 GII.4 variant Norovirus strain from Ireland.

dc.contributor.authorKearney, Karen
dc.contributor.authorMenton, John
dc.contributor.authorMorgan, John G
dc.date.accessioned2010-04-06T10:17:54Z
dc.date.available2010-04-06T10:17:54Z
dc.date.issued2007
dc.identifier.citationCarlow virus, a 2002 GII.4 variant Norovirus strain from Ireland. 2007, 4:61 Virol. J.en
dc.identifier.issn1743-422X
dc.identifier.pmid17567897
dc.identifier.doi10.1186/1743-422X-4-61
dc.identifier.urihttp://hdl.handle.net/10147/95629
dc.description.abstractBACKGROUND: Noroviruses are the leading cause of infectious non-bacterial gastroenteritis in Ireland (population 4 million). Due to the number of outbreaks, its massive impact on the Irish health service and its seasonality, Norovirus has gained public notoriety as The Winter Vomiting Bug. The increase in cases in Ireland in the 2002-2003 season coincided with the emergence of two new Genogroup II genotype 4 variant clusters of Norovirus worldwide. RESULTS: Little research has been done on the epidemiology or molecular biology of Norovirus strains in Ireland. In an effort to combat this discrepancy, we cloned a full length human norovirus genome as a cDNA clone (J3) which can produce full length transcripts in vitro. A polymerase mutant cDNA clone (X1), in addition to a sub genomic cDNA clone (1A) were produced for use in future work. Carlow virus (Hu/NoV/GII/Carlow/2002/Ire) genome is 7559 nts in length, excluding the 3-end poly A tail and represents the first Norovirus strain from Ireland to be sequenced. CONCLUSION: Carlow virus is a member of the Farmington Hills variant cluster of Genogroup II genotype 4 noroviruses.
dc.language.isoenen
dc.subject.meshDNA, Complementary
dc.subject.meshDNA, Viral
dc.subject.meshDisease Outbreaks
dc.subject.meshGastroenteritis
dc.subject.meshGenetic Variation
dc.subject.meshGenome, Viral
dc.subject.meshGenotype
dc.subject.meshHumans
dc.subject.meshIreland
dc.subject.meshMutation
dc.subject.meshNorovirus
dc.subject.meshPolymerase Chain Reaction
dc.subject.meshRNA, Viral
dc.subject.meshTranscription, Genetic
dc.titleCarlow virus, a 2002 GII.4 variant Norovirus strain from Ireland.en
dc.contributor.departmentLab 439, Department of Microbiology, University College Cork, Cork, Ireland. kearney_karen@hotmail.comen
dc.identifier.journalVirology journalen
refterms.dateFOA2018-09-03T10:43:13Z
html.description.abstractBACKGROUND: Noroviruses are the leading cause of infectious non-bacterial gastroenteritis in Ireland (population 4 million). Due to the number of outbreaks, its massive impact on the Irish health service and its seasonality, Norovirus has gained public notoriety as The Winter Vomiting Bug. The increase in cases in Ireland in the 2002-2003 season coincided with the emergence of two new Genogroup II genotype 4 variant clusters of Norovirus worldwide. RESULTS: Little research has been done on the epidemiology or molecular biology of Norovirus strains in Ireland. In an effort to combat this discrepancy, we cloned a full length human norovirus genome as a cDNA clone (J3) which can produce full length transcripts in vitro. A polymerase mutant cDNA clone (X1), in addition to a sub genomic cDNA clone (1A) were produced for use in future work. Carlow virus (Hu/NoV/GII/Carlow/2002/Ire) genome is 7559 nts in length, excluding the 3-end poly A tail and represents the first Norovirus strain from Ireland to be sequenced. CONCLUSION: Carlow virus is a member of the Farmington Hills variant cluster of Genogroup II genotype 4 noroviruses.


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