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    Carlow virus, a 2002 GII.4 variant Norovirus strain from Ireland.

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    Authors
    Kearney, Karen
    Menton, John
    Morgan, John G
    Affiliation
    Lab 439, Department of Microbiology, University College Cork, Cork, Ireland. kearney_karen@hotmail.com
    Issue Date
    2007
    MeSH
    DNA, Complementary
    DNA, Viral
    Disease Outbreaks
    Gastroenteritis
    Genetic Variation
    Genome, Viral
    Genotype
    Humans
    Ireland
    Mutation
    Norovirus
    Polymerase Chain Reaction
    RNA, Viral
    Transcription, Genetic
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    Citation
    Carlow virus, a 2002 GII.4 variant Norovirus strain from Ireland. 2007, 4:61 Virol. J.
    Journal
    Virology journal
    URI
    http://hdl.handle.net/10147/95629
    DOI
    10.1186/1743-422X-4-61
    PubMed ID
    17567897
    Abstract
    BACKGROUND: Noroviruses are the leading cause of infectious non-bacterial gastroenteritis in Ireland (population 4 million). Due to the number of outbreaks, its massive impact on the Irish health service and its seasonality, Norovirus has gained public notoriety as The Winter Vomiting Bug. The increase in cases in Ireland in the 2002-2003 season coincided with the emergence of two new Genogroup II genotype 4 variant clusters of Norovirus worldwide. RESULTS: Little research has been done on the epidemiology or molecular biology of Norovirus strains in Ireland. In an effort to combat this discrepancy, we cloned a full length human norovirus genome as a cDNA clone (J3) which can produce full length transcripts in vitro. A polymerase mutant cDNA clone (X1), in addition to a sub genomic cDNA clone (1A) were produced for use in future work. Carlow virus (Hu/NoV/GII/Carlow/2002/Ire) genome is 7559 nts in length, excluding the 3-end poly A tail and represents the first Norovirus strain from Ireland to be sequenced. CONCLUSION: Carlow virus is a member of the Farmington Hills variant cluster of Genogroup II genotype 4 noroviruses.
    Language
    en
    ISSN
    1743-422X
    ae974a485f413a2113503eed53cd6c53
    10.1186/1743-422X-4-61
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