Role of subtyping in detecting Salmonella cross contamination in the laboratory.
Affiliation
National Salmonella Reference Laboratory, Department of Medical Microbiology, Galway University Hospitals, Galway, Ireland. niall.delappe@hse.ieIssue Date
2009MeSH
Bacteriophage TypingElectrophoresis, Gel, Pulsed-Field
Equipment Contamination
Food Contamination
Food Microbiology
Laboratories
Microbial Sensitivity Tests
Salmonella
Serotyping
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Role of subtyping in detecting Salmonella cross contamination in the laboratory. 2009, 9:155 BMC Microbiol.Journal
BMC microbiologyDOI
10.1186/1471-2180-9-155PubMed ID
19646244Abstract
BACKGROUND: With the exception of M. tuberculosis, little has been published on the problems of cross-contamination in bacteriology laboratories. We performed a retrospective analysis of subtyping data from the National Salmonella Reference Laboratory (Ireland) from 2000-2007 to identify likely incidents of laboratory cross contamination. METHODS: Serotyping and antimicrobial susceptibility testing was performed on all Salmonella isolates received in the NSRL. Phage typing was performed on all S. Typhimurium and S. Enteritidis isolates while multi-locus variance analysis (MLVA) was performed on selected S. Typhimurium isolates. Pulsed field gel electrophoresis (PFGE) using the PulseNet standard protocol was performed on selected isolates of various serovars. RESULTS: Twenty-three incidents involving fifty-six isolates were identified as likely to represent cross contamination. The probable sources of contamination identified were the laboratory positive control isolate (n = 13), other test isolates (n = 9) or proficiency test samples (n = 1). CONCLUSION: The scale of laboratory cross-contamination in bacteriology is most likely under recognized. Testing laboratories should be aware of the potential for cross-contamination, regularly review protocols to minimize its occurrence and consider it as a possibility when unexpected results are obtained.Language
enISSN
1471-2180ae974a485f413a2113503eed53cd6c53
10.1186/1471-2180-9-155
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