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    Role of subtyping in detecting Salmonella cross contamination in the laboratory.

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    Authors
    De Lappe, Niall
    Connor, Jean O
    Doran, Geraldine
    Devane, Genevieve
    Cormican, Martin
    Affiliation
    National Salmonella Reference Laboratory, Department of Medical Microbiology, Galway University Hospitals, Galway, Ireland. niall.delappe@hse.ie
    Issue Date
    2009
    MeSH
    Bacteriophage Typing
    Electrophoresis, Gel, Pulsed-Field
    Equipment Contamination
    Food Contamination
    Food Microbiology
    Laboratories
    Microbial Sensitivity Tests
    Salmonella
    Serotyping
    
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    Citation
    Role of subtyping in detecting Salmonella cross contamination in the laboratory. 2009, 9:155 BMC Microbiol.
    Journal
    BMC microbiology
    URI
    http://hdl.handle.net/10147/95263
    DOI
    10.1186/1471-2180-9-155
    PubMed ID
    19646244
    Abstract
    BACKGROUND: With the exception of M. tuberculosis, little has been published on the problems of cross-contamination in bacteriology laboratories. We performed a retrospective analysis of subtyping data from the National Salmonella Reference Laboratory (Ireland) from 2000-2007 to identify likely incidents of laboratory cross contamination. METHODS: Serotyping and antimicrobial susceptibility testing was performed on all Salmonella isolates received in the NSRL. Phage typing was performed on all S. Typhimurium and S. Enteritidis isolates while multi-locus variance analysis (MLVA) was performed on selected S. Typhimurium isolates. Pulsed field gel electrophoresis (PFGE) using the PulseNet standard protocol was performed on selected isolates of various serovars. RESULTS: Twenty-three incidents involving fifty-six isolates were identified as likely to represent cross contamination. The probable sources of contamination identified were the laboratory positive control isolate (n = 13), other test isolates (n = 9) or proficiency test samples (n = 1). CONCLUSION: The scale of laboratory cross-contamination in bacteriology is most likely under recognized. Testing laboratories should be aware of the potential for cross-contamination, regularly review protocols to minimize its occurrence and consider it as a possibility when unexpected results are obtained.
    Language
    en
    ISSN
    1471-2180
    ae974a485f413a2113503eed53cd6c53
    10.1186/1471-2180-9-155
    Scopus Count
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    Journal articles & published research

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