• Login
    View Item 
    •   Home
    • Irish Health Organisations
    • Publications
    • View Item
    •   Home
    • Irish Health Organisations
    • Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Map of Submissions

    Home Page
    UlsterN
    5118
    UlsterS
    5118
    Connacht
    1710
    Munster
    58
    Leinster
    467

    Browse

    All of Lenus, The Irish Health RepositoryCommunitiesTitleAuthorsDate publishedSubjectsThis CollectionTitleAuthorsDate publishedSubjects

    My Account

    LoginRegister

    About

    About LenusDirectory of Open Access JournalsOpen Access Publishing GuideNational Health Library & Knowledge ServiceGuide to Publishers' PoliciesFAQsTerms and ConditionsVision StatementORCID Unique identifiers for ResearchersHSE position statement on Open AccessNational Open Research Forum (NORF)Zenodo (European Open Research repository)

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Bicaudal is a conserved substrate for Drosophila and mammalian caspases and is essential for cell survival.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    19330035.pdf
    Size:
    1.325Mb
    Format:
    PDF
    Download
    Authors
    Creagh, Emma M
    Brumatti, Gabriela
    Sheridan, Clare
    Duriez, Patrick J
    Taylor, Rebecca C
    Cullen, Sean P
    Adrain, Colin
    Martin, Seamus J
    Affiliation
    Molecular Cell Biology Laboratory, Department of Genetics, Smurfit Institute, Trinity College, Dublin, Ireland [corrected]
    Issue Date
    2009
    MeSH
    Animals
    Apoptosis
    Caspases
    Cell Line
    Cell Survival
    Drosophila
    Drosophila Proteins
    Humans
    Mammals
    Proteomics
    RNA-Binding Proteins
    Species Specificity
    Substrate Specificity
    Show allShow less
    
    Metadata
    Show full item record
    Citation
    Bicaudal is a conserved substrate for Drosophila and mammalian caspases and is essential for cell survival. 2009, 4 (3):e5055 PLoS ONE
    Journal
    PloS one
    URI
    http://hdl.handle.net/10147/94731
    DOI
    10.1371/journal.pone.0005055
    PubMed ID
    19330035
    Abstract
    Members of the caspase family of cysteine proteases coordinate cell death through restricted proteolysis of diverse protein substrates and play a conserved role in apoptosis from nematodes to man. However, while numerous substrates for the mammalian cell death-associated caspases have now been described, few caspase substrates have been identified in other organisms. Here, we have utilized a proteomics-based approach to identify proteins that are cleaved by caspases during apoptosis in Drosophila D-Mel2 cells, a subline of the Schneider S2 cell line. This approach identified multiple novel substrates for the fly caspases and revealed that bicaudal/betaNAC is a conserved substrate for Drosophila and mammalian caspases. RNAi-mediated silencing of bicaudal expression in Drosophila D-Mel2 cells resulted in a block to proliferation, followed by spontaneous apoptosis. Similarly, silencing of expression of the mammalian bicaudal homologue, betaNAC, in HeLa, HEK293T, MCF-7 and MRC5 cells also resulted in spontaneous apoptosis. These data suggest that bicaudal/betaNAC is essential for cell survival and is a conserved target of caspases from flies to man.
    Language
    en
    ISSN
    1932-6203
    ae974a485f413a2113503eed53cd6c53
    10.1371/journal.pone.0005055
    Scopus Count
    Collections
    Publications

    entitlement

     
    Health Library Ireland | Health Service Executive | Jervis House, Jervis Street | Republic of Ireland | Eircode: D01 W596
    lenus@hse.ie | Tel: +353-1-7786275
    DSpace software copyright © 2002-2017  DuraSpace
    Contact Us | Disclaimer
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.