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dc.contributor.authorFang, Fang
dc.contributor.authorLi, Yin
dc.contributor.authorBumann, Mario
dc.contributor.authorRaftis, Emma J
dc.contributor.authorCasey, Pat G
dc.contributor.authorCooney, Jakki C
dc.contributor.authorWalsh, Martin A
dc.contributor.authorO'Toole, Paul W
dc.date.accessioned2010-03-12T15:37:24Z
dc.date.available2010-03-12T15:37:24Z
dc.date.issued2009-09
dc.identifier.citationAllelic variation of bile salt hydrolase genes in Lactobacillus salivarius does not determine bile resistance levels. 2009, 191 (18):5743-57 J. Bacteriol.en
dc.identifier.issn1098-5530
dc.identifier.pmid19592587
dc.identifier.doi10.1128/JB.00506-09
dc.identifier.urihttp://hdl.handle.net/10147/94172
dc.description.abstractCommensal lactobacilli frequently produce bile salt hydrolase (Bsh) enzymes whose roles in intestinal survival are unclear. Twenty-six Lactobacillus salivarius strains from different sources all harbored a bsh1 allele on their respective megaplasmids. This allele was related to the plasmid-borne bsh1 gene of the probiotic strain UCC118. A second locus (bsh2) was found in the chromosomes of two strains that had higher bile resistance levels. Four Bsh1-encoding allele groups were identified, defined by truncations or deletions involving a conserved residue. In vitro analyses showed that this allelic variation was correlated with widely varying bile deconjugation phenotypes. Despite very low activity of the UCC118 Bsh1 enzyme, a mutant lacking this protein had significantly lower bile resistance, both in vitro and during intestinal transit in mice. However, the overall bile resistance phenotype of this and other strains was independent of the bsh1 allele type. Analysis of the L. salivarius transcriptome upon exposure to bile and cholate identified a multiplicity of stress response proteins and putative efflux proteins that appear to broadly compensate for, or mask, the effects of allelic variation of bsh genes. Bsh enzymes with different bile-degrading kinetics, though apparently not the primary determinants of bile resistance in L. salivarius, may have additional biological importance because of varying effects upon bile as a signaling molecule in the host.
dc.description.abstractCommensal lactobacilli frequently produce bile salt hydrolase (Bsh) enzymes whose roles in intestinal survival are unclear. Twenty-six Lactobacillus salivarius strains from different sources all harbored a bsh1 allele on their respective megaplasmids. This allele was related to the plasmid-borne bsh1 gene of the probiotic strain UCC118. A second locus (bsh2) was found in the chromosomes of two strains that had higher bile resistance levels. Four Bsh1-encoding allele groups were identified, defined by truncations or deletions involving a conserved residue. In vitro analyses showed that this allelic variation was correlated with widely varying bile deconjugation phenotypes. Despite very low activity of the UCC118 Bsh1 enzyme, a mutant lacking this protein had significantly lower bile resistance, both in vitro and during intestinal transit in mice. However, the overall bile resistance phenotype of this and other strains was independent of the bsh1 allele type. Analysis of the L. salivarius transcriptome upon exposure to bile and cholate identified a multiplicity of stress response proteins and putative efflux proteins that appear to broadly compensate for, or mask, the effects of allelic variation of bsh genes. Bsh enzymes with different bile-degrading kinetics, though apparently not the primary determinants of bile resistance in L. salivarius, may have additional biological importance because of varying effects upon bile as a signaling molecule in the host.
dc.language.isoenen
dc.subject.meshAlleles
dc.subject.meshAlleles
dc.subject.meshAmidohydrolases
dc.subject.meshAmidohydrolases
dc.subject.meshAnimals
dc.subject.meshAnimals
dc.subject.meshBacterial Proteins
dc.subject.meshBacterial Proteins
dc.subject.meshBile Acids and Salts
dc.subject.meshBile Acids and Salts
dc.subject.meshDrug Resistance, Bacterial
dc.subject.meshDrug Resistance, Bacterial
dc.subject.meshGenetic Variation
dc.subject.meshGenetic Variation
dc.subject.meshHumans
dc.subject.meshHumans
dc.subject.meshIntestines
dc.subject.meshIntestines
dc.subject.meshLactobacillus
dc.subject.meshLactobacillus
dc.subject.meshMice
dc.subject.meshMice
dc.subject.meshMicrobial Sensitivity Tests
dc.subject.meshMicrobial Sensitivity Tests
dc.subject.meshMolecular Sequence Data
dc.subject.meshMolecular Sequence Data
dc.subject.meshMutation
dc.subject.meshMutation
dc.subject.meshOligonucleotide Array Sequence Analysis
dc.subject.meshOligonucleotide Array Sequence Analysis
dc.subject.meshPhylogeny
dc.subject.meshPhylogeny
dc.titleAllelic variation of bile salt hydrolase genes in Lactobacillus salivarius does not determine bile resistance levels.en
dc.contributor.departmentDepartment of Microbiology, University College Cork, Cork, Ireland.en
dc.identifier.journalJournal of bacteriologyen
refterms.dateFOA2018-09-03T10:38:00Z
html.description.abstractCommensal lactobacilli frequently produce bile salt hydrolase (Bsh) enzymes whose roles in intestinal survival are unclear. Twenty-six Lactobacillus salivarius strains from different sources all harbored a bsh1 allele on their respective megaplasmids. This allele was related to the plasmid-borne bsh1 gene of the probiotic strain UCC118. A second locus (bsh2) was found in the chromosomes of two strains that had higher bile resistance levels. Four Bsh1-encoding allele groups were identified, defined by truncations or deletions involving a conserved residue. In vitro analyses showed that this allelic variation was correlated with widely varying bile deconjugation phenotypes. Despite very low activity of the UCC118 Bsh1 enzyme, a mutant lacking this protein had significantly lower bile resistance, both in vitro and during intestinal transit in mice. However, the overall bile resistance phenotype of this and other strains was independent of the bsh1 allele type. Analysis of the L. salivarius transcriptome upon exposure to bile and cholate identified a multiplicity of stress response proteins and putative efflux proteins that appear to broadly compensate for, or mask, the effects of allelic variation of bsh genes. Bsh enzymes with different bile-degrading kinetics, though apparently not the primary determinants of bile resistance in L. salivarius, may have additional biological importance because of varying effects upon bile as a signaling molecule in the host.
html.description.abstractCommensal lactobacilli frequently produce bile salt hydrolase (Bsh) enzymes whose roles in intestinal survival are unclear. Twenty-six Lactobacillus salivarius strains from different sources all harbored a bsh1 allele on their respective megaplasmids. This allele was related to the plasmid-borne bsh1 gene of the probiotic strain UCC118. A second locus (bsh2) was found in the chromosomes of two strains that had higher bile resistance levels. Four Bsh1-encoding allele groups were identified, defined by truncations or deletions involving a conserved residue. In vitro analyses showed that this allelic variation was correlated with widely varying bile deconjugation phenotypes. Despite very low activity of the UCC118 Bsh1 enzyme, a mutant lacking this protein had significantly lower bile resistance, both in vitro and during intestinal transit in mice. However, the overall bile resistance phenotype of this and other strains was independent of the bsh1 allele type. Analysis of the L. salivarius transcriptome upon exposure to bile and cholate identified a multiplicity of stress response proteins and putative efflux proteins that appear to broadly compensate for, or mask, the effects of allelic variation of bsh genes. Bsh enzymes with different bile-degrading kinetics, though apparently not the primary determinants of bile resistance in L. salivarius, may have additional biological importance because of varying effects upon bile as a signaling molecule in the host.


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