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    Allelic variation of bile salt hydrolase genes in Lactobacillus salivarius does not determine bile resistance levels.

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    Authors
    Fang, Fang
    Li, Yin
    Bumann, Mario
    Raftis, Emma J
    Casey, Pat G
    Cooney, Jakki C
    Walsh, Martin A
    O'Toole, Paul W
    Affiliation
    Department of Microbiology, University College Cork, Cork, Ireland.
    Issue Date
    2009-09
    MeSH
    Alleles
    Alleles
    Amidohydrolases
    Amidohydrolases
    Animals
    Animals
    Bacterial Proteins
    Bacterial Proteins
    Bile Acids and Salts
    Bile Acids and Salts
    Drug Resistance, Bacterial
    Drug Resistance, Bacterial
    Genetic Variation
    Genetic Variation
    Humans
    Humans
    Intestines
    Intestines
    Lactobacillus
    Lactobacillus
    Mice
    Mice
    Microbial Sensitivity Tests
    Microbial Sensitivity Tests
    Molecular Sequence Data
    Molecular Sequence Data
    Mutation
    Mutation
    Oligonucleotide Array Sequence Analysis
    Oligonucleotide Array Sequence Analysis
    Phylogeny
    Phylogeny
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    Citation
    Allelic variation of bile salt hydrolase genes in Lactobacillus salivarius does not determine bile resistance levels. 2009, 191 (18):5743-57 J. Bacteriol.
    Journal
    Journal of bacteriology
    URI
    http://hdl.handle.net/10147/94172
    DOI
    10.1128/JB.00506-09
    PubMed ID
    19592587
    Abstract
    Commensal lactobacilli frequently produce bile salt hydrolase (Bsh) enzymes whose roles in intestinal survival are unclear. Twenty-six Lactobacillus salivarius strains from different sources all harbored a bsh1 allele on their respective megaplasmids. This allele was related to the plasmid-borne bsh1 gene of the probiotic strain UCC118. A second locus (bsh2) was found in the chromosomes of two strains that had higher bile resistance levels. Four Bsh1-encoding allele groups were identified, defined by truncations or deletions involving a conserved residue. In vitro analyses showed that this allelic variation was correlated with widely varying bile deconjugation phenotypes. Despite very low activity of the UCC118 Bsh1 enzyme, a mutant lacking this protein had significantly lower bile resistance, both in vitro and during intestinal transit in mice. However, the overall bile resistance phenotype of this and other strains was independent of the bsh1 allele type. Analysis of the L. salivarius transcriptome upon exposure to bile and cholate identified a multiplicity of stress response proteins and putative efflux proteins that appear to broadly compensate for, or mask, the effects of allelic variation of bsh genes. Bsh enzymes with different bile-degrading kinetics, though apparently not the primary determinants of bile resistance in L. salivarius, may have additional biological importance because of varying effects upon bile as a signaling molecule in the host.
    Commensal lactobacilli frequently produce bile salt hydrolase (Bsh) enzymes whose roles in intestinal survival are unclear. Twenty-six Lactobacillus salivarius strains from different sources all harbored a bsh1 allele on their respective megaplasmids. This allele was related to the plasmid-borne bsh1 gene of the probiotic strain UCC118. A second locus (bsh2) was found in the chromosomes of two strains that had higher bile resistance levels. Four Bsh1-encoding allele groups were identified, defined by truncations or deletions involving a conserved residue. In vitro analyses showed that this allelic variation was correlated with widely varying bile deconjugation phenotypes. Despite very low activity of the UCC118 Bsh1 enzyme, a mutant lacking this protein had significantly lower bile resistance, both in vitro and during intestinal transit in mice. However, the overall bile resistance phenotype of this and other strains was independent of the bsh1 allele type. Analysis of the L. salivarius transcriptome upon exposure to bile and cholate identified a multiplicity of stress response proteins and putative efflux proteins that appear to broadly compensate for, or mask, the effects of allelic variation of bsh genes. Bsh enzymes with different bile-degrading kinetics, though apparently not the primary determinants of bile resistance in L. salivarius, may have additional biological importance because of varying effects upon bile as a signaling molecule in the host.
    Language
    en
    ISSN
    1098-5530
    ae974a485f413a2113503eed53cd6c53
    10.1128/JB.00506-09
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