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A synthetic consortium of 100 gut commensals modulates the composition and function in a colon model of the microbiome of elderly subjects.

dc.contributor.authorPerez, Marta
dc.contributor.authorNtemiri, Alexandra
dc.contributor.authorTan, Huizi
dc.contributor.authorHarris, Hugh M B
dc.contributor.authorRoager, Henrik M
dc.contributor.authorRibière, Céline
dc.contributor.authorO'Toole, Paul W
dc.date.accessioned2025-04-22T09:55:13Z
dc.date.available2025-04-22T09:55:13Z
dc.date.issued2021-01
dc.identifier.pmid33993839
dc.identifier.doi10.1080/19490976.2021.1919464
dc.identifier.urihttp://hdl.handle.net/10147/644916
dc.descriptionAdministration of cultured gut isolates holds promise for modulating the altered composition and function of the microbiota in older subjects, and for promoting their health. From among 692 initial isolates, we selected 100 gut commensal strains (MCC100) based on emulating the gut microbiota of healthy subjects, and retaining strain diversity within selected species. MCC100 susceptibility to seven antibiotics was determined, and their genomes were screened for virulence factor, antimicrobial resistance and bacteriocin genes. Supplementation of healthy and frail elderly microbiota types with the MCC100 in an in vitro colon model increased alpha-diversity, raised relative abundance of taxa including Blautia luti, Bacteroides fragilis, and Sutterella wadsworthensis; and introduced taxa such as Bifidobacterium spp. Microbiota changes correlated with higher levels of branched chain amino acids, which are health-associated in elderly. The study establishes that the MCC100 consortium can modulate older subjects' microbiota composition and associated metabolome in vitro, paving the way for pre-clinical and human trials.en_US
dc.language.isoenen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectBCAAen_US
dc.subjectgut microbiomeen_US
dc.subjectalpha-diversityen_US
dc.subjectANTIBIOTICSen_US
dc.subjectbranched chain amino acidsen_US
dc.subjectconsortiumen_US
dc.subjectElderlyen_US
dc.subjectsynthetic microbiomeen_US
dc.titleA synthetic consortium of 100 gut commensals modulates the composition and function in a colon model of the microbiome of elderly subjects.en_US
dc.typeArticleen_US
dc.identifier.eissn1949-0984
dc.identifier.journalGut microbesen_US
dc.source.journaltitleGut microbes
dc.source.volume13
dc.source.issue1
dc.source.beginpage1
dc.source.endpage19
refterms.dateFOA2025-04-22T09:55:15Z
dc.source.countryUnited States


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International