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dc.contributor.authorCassedy, A
dc.contributor.authorParle-McDermott, A
dc.contributor.authorO'Kennedy, R
dc.date.accessioned2022-02-25T17:17:49Z
dc.date.available2022-02-25T17:17:49Z
dc.date.issued2021-04-20
dc.identifier.issn2296-889X
dc.identifier.pmid33959631
dc.identifier.doi10.3389/fmolb.2021.637559
dc.identifier.urihttp://hdl.handle.net/10147/631573
dc.descriptionViruses are ubiquitous in the environment. While many impart no deleterious effects on their hosts, several are major pathogens. This risk of pathogenicity, alongside the fact that many viruses can rapidly mutate highlights the need for suitable, rapid diagnostic measures. This review provides a critical analysis of widely used methods and examines their advantages and limitations. Currently, nucleic-acid detection and immunoassay methods are among the most popular means for quickly identifying viral infection directly from source. Nucleic acid-based detection generally offers high sensitivity, but can be time-consuming, costly, and require trained staff. The use of isothermal-based amplification systems for detection could aid in the reduction of results turnaround and equipment-associated costs, making them appealing for point-of-use applications, or when high volume/fast turnaround testing is required. Alternatively, immunoassays offer robustness and reduced costs. Furthermore, some immunoassay formats, such as those using lateral-flow technology, can generate results very rapidly. However, immunoassays typically cannot achieve comparable sensitivity to nucleic acid-based detection methods. Alongside these methods, the application of next-generation sequencing can provide highly specific results. In addition, the ability to sequence large numbers of viral genomes would provide researchers with enhanced information and assist in tracing infections.en_US
dc.language.isoenen_US
dc.rightsCopyright © 2021 Cassedy, Parle-McDermott and O’Kennedy.
dc.subjectimmunoassayen_US
dc.subjectisothermal amplificationen_US
dc.subjectnext generation sequencingen_US
dc.subjectnucleic-acid detectionen_US
dc.subjectSAMPLINGen_US
dc.subjectVIRUSen_US
dc.titleVirus Detection: A Review of the Current and Emerging Molecular and Immunological Methods.en_US
dc.typeArticleen_US
dc.identifier.journalFrontiers in molecular biosciencesen_US
dc.source.journaltitleFrontiers in molecular biosciences
dc.source.volume8
dc.source.beginpage637559
dc.source.endpage
refterms.dateFOA2022-02-25T17:17:51Z
dc.source.countrySwitzerland


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