Association of synovial tissue polyfunctional T-cells with DAPSA in psoriatic arthritis.
Authors
Wade, Sarah MCanavan, Mary
McGarry, Trudy
Low, Candice
Wade, Siobhan C
Mullan, Ronan H
Veale, Douglas J
Fearon, Ursula
Issue Date
2019-01-09Keywords
polyfunctional t-cellspsoriatic arthritis
synovial tissue
CELL BIOLOGY
Metadata
Show full item recordJournal
Annals of the rheumatic diseasesDOI
10.1136/annrheumdis-2018-214138PubMed ID
30626658Abstract
PsA synovial tissue infiltrating CD4+ T-cells expressed higher levels of interleukin (IL)-17A, interferon gamma (IFN-γ), GM-CSF and CD161, with parallel enrichment of Th1, Th17 and exTh17 T-helper subsets (all p<0.05). Interestingly, a significant proportion of synovial T-cell subsets were triple-positive for GM-CSF, tumour necrosis factor (-TNF), -IL-17 or IFN-γ compared with matched blood (all p<0.05). Importantly, frequencies of polyfunctional T-cells correlated with DAPSA: Th1-GM-CSF+/TNF+/IFN-γ+ (r=0.7, p<0.01), Th17-GM-CSF+/TNF+/IL-17+ (r=0.6, p<0.057) and exTh17-GM-CSF+/TNF+/IFN-γ+ (r=0.7, p=0.0096), with no associations observed for single cytokine-producing T-cells. Following ex vivo culture of PsA synovial tissue cell suspensions, polyfunctional GM-CSF+TNFα+IL-17A+ or/IFN-γ+-producing T-cells (p<0.05), but not single cytokine-producing T-cells, were inhibited with a PDE4 inhibitor. Conclusion: These data demonstrate enrichment of polyfunctional T-cells in PsA synovial tissue which were strongly associated with DAPSA and ex vivo therapeutic response.Item Type
ArticleOther
Language
enEISSN
1468-2060ae974a485f413a2113503eed53cd6c53
10.1136/annrheumdis-2018-214138
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