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Isolation and gene expression profiling of intestinal epithelial cells: crypt isolation by calcium chelation from in vivo samples.

dc.contributor.authorBalfe, Aine
dc.contributor.authorLennon, Grainne
dc.contributor.authorLavelle, Aonghus
dc.contributor.authorDocherty, Neil G
dc.contributor.authorCoffey, J Calvin
dc.contributor.authorSheahan, Kieran
dc.contributor.authorWinter, Desmond C
dc.contributor.authorO'Connell, P Ronan
dc.date.accessioned2018-02-16T10:41:34Z
dc.date.available2018-02-16T10:41:34Z
dc.date.issued2018
dc.identifier.citationIsolation and gene expression profiling of intestinal epithelial cells: crypt isolation by calcium chelation from in vivo samples. 2018, 11:29-37 Clin Exp Gastroenterolen
dc.identifier.issn1178-7023
dc.identifier.pmid29391821
dc.identifier.doi10.2147/CEG.S145224
dc.identifier.urihttp://hdl.handle.net/10147/622795
dc.description.abstractThe epithelial layer within the colon represents a physical barrier between the luminal contents and its underlying mucosa. It plays a pivotal role in mucosal homeostasis, and both tolerance and anti-pathogenic immune responses. Identifying signals of inflammation initiation and responses to stimuli from within the epithelial layer is critical to understanding the molecular pathways underlying disease pathology. This study validated a method to isolate and analyze epithelial populations, enabling investigations of epithelial function and response in a variety of disease setting.
dc.language.isoenen
dc.publisherClinical and Experimental Gastroenterologyen
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pubmed/29391821en
dc.rightsArchived with thanks to Clinical and experimental gastroenterologyen
dc.subjectIMMUNE SYSTEMen
dc.subjectGUT MICROBIAen
dc.subject.otherINTESTINEen
dc.titleIsolation and gene expression profiling of intestinal epithelial cells: crypt isolation by calcium chelation from in vivo samples.en
dc.typeArticleen
dc.identifier.journalClinical and experimental gastroenterologyen
dc.description.fundingSFI Science Foundation Irelanden
dc.description.provinceLeinsteren
dc.description.peer-reviewpeer-reviewen
refterms.dateFOA2018-08-28T01:42:40Z
html.description.abstractThe epithelial layer within the colon represents a physical barrier between the luminal contents and its underlying mucosa. It plays a pivotal role in mucosal homeostasis, and both tolerance and anti-pathogenic immune responses. Identifying signals of inflammation initiation and responses to stimuli from within the epithelial layer is critical to understanding the molecular pathways underlying disease pathology. This study validated a method to isolate and analyze epithelial populations, enabling investigations of epithelial function and response in a variety of disease setting.


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