Isolation and gene expression profiling of intestinal epithelial cells: crypt isolation by calcium chelation from in vivo samples.
Docherty, Neil G
Coffey, J Calvin
Winter, Desmond C
O'Connell, P Ronan
Local subject classificationINTESTINE
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CitationIsolation and gene expression profiling of intestinal epithelial cells: crypt isolation by calcium chelation from in vivo samples. 2018, 11:29-37 Clin Exp Gastroenterol
JournalClinical and experimental gastroenterology
AbstractThe epithelial layer within the colon represents a physical barrier between the luminal contents and its underlying mucosa. It plays a pivotal role in mucosal homeostasis, and both tolerance and anti-pathogenic immune responses. Identifying signals of inflammation initiation and responses to stimuli from within the epithelial layer is critical to understanding the molecular pathways underlying disease pathology. This study validated a method to isolate and analyze epithelial populations, enabling investigations of epithelial function and response in a variety of disease setting.