Isolation and gene expression profiling of intestinal epithelial cells: crypt isolation by calcium chelation from in vivo samples.
Authors
Balfe, AineLennon, Grainne
Lavelle, Aonghus
Docherty, Neil G
Coffey, J Calvin
Sheahan, Kieran
Winter, Desmond C
O'Connell, P Ronan
Issue Date
2018Keywords
IMMUNE SYSTEMGUT MICROBIA
Local subject classification
INTESTINE
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Isolation and gene expression profiling of intestinal epithelial cells: crypt isolation by calcium chelation from in vivo samples. 2018, 11:29-37 Clin Exp GastroenterolJournal
Clinical and experimental gastroenterologyDOI
10.2147/CEG.S145224PubMed ID
29391821Additional Links
https://www.ncbi.nlm.nih.gov/pubmed/29391821Abstract
The epithelial layer within the colon represents a physical barrier between the luminal contents and its underlying mucosa. It plays a pivotal role in mucosal homeostasis, and both tolerance and anti-pathogenic immune responses. Identifying signals of inflammation initiation and responses to stimuli from within the epithelial layer is critical to understanding the molecular pathways underlying disease pathology. This study validated a method to isolate and analyze epithelial populations, enabling investigations of epithelial function and response in a variety of disease setting.Item Type
ArticleLanguage
enISSN
1178-7023ae974a485f413a2113503eed53cd6c53
10.2147/CEG.S145224
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