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dc.contributor.authorNugent, Sharon M E
dc.contributor.authorMothersill, Carmel E
dc.contributor.authorSeymour, Colin
dc.contributor.authorMcClean, Brendan
dc.contributor.authorLyng, Fiona M
dc.contributor.authorMurphy, James E J
dc.date.accessioned2017-05-29T14:42:44Z
dc.date.available2017-05-29T14:42:44Z
dc.date.issued2007-07
dc.identifier.citationIncreased mitochondrial mass in cells with functionally compromised mitochondria after exposure to both direct gamma radiation and bystander factors. 2007, 168 (1):134-42 Radiat. Res.en
dc.identifier.issn0033-7587
dc.identifier.pmid17722997
dc.identifier.doi10.1667/RR0769.1
dc.identifier.urihttp://hdl.handle.net/10147/621398
dc.description.abstractThe bystander effect describes radiation-like damage in unirradiated cells either in the vicinity of irradiated cells or exposed to medium from irradiated cells. This study aimed to further characterize the poorly understood mitochondrial response to both direct irradiation and bystander factor(s) in human keratinocytes (HPV-G) and Chinese hamster ovarian cells (CHO-K1). Oxygen consumption rates were determined during periods of state 4, state 3 and uncoupled respiration. Mitochondrial mass was determined using MitoTracker FM. CHO-K1 cells showed significantly reduced oxygen consumption rates 4 h after exposure to 5 Gy direct radiation and irradiated cell conditioned medium (ICCM) and an apparent recovery 12-24 h later. The apparent recovery was likely due to the substantial increase in mitochondrial mass observed in these cells as soon as 4 h after exposure. HPV-G cells, on the other hand, showed a sustained increase in oxygen consumption rates after ICCM exposure and a transient increase 4 h after exposure to 5 Gy direct radiation. A significant increase in mitochondrial mass per HPV-G cell was observed after exposure to both direct radiation and ICCM. These findings are indicative of a stress response to mitochondrial dysfunction that increases the number of mitochondria per cell.
dc.language.isoenen
dc.rightsArchived with thanks to Radiation researchen
dc.subjectRADIOTHERAPYen
dc.subjectRADIATIONen
dc.subject.meshAnimals
dc.subject.meshBystander Effect
dc.subject.meshCell Line
dc.subject.meshCricetinae
dc.subject.meshGamma Rays
dc.subject.meshHumans
dc.subject.meshMitochondria
dc.subject.meshMitochondrial Size
dc.subject.meshOxygen
dc.titleIncreased mitochondrial mass in cells with functionally compromised mitochondria after exposure to both direct gamma radiation and bystander factors.en
dc.typeArticleen
dc.identifier.journalRadiation researchen
dc.description.fundingNo fundingen
dc.description.provinceLeinsteren
dc.description.peer-reviewpeer-reviewen
refterms.dateFOA2018-08-27T21:26:13Z
html.description.abstractThe bystander effect describes radiation-like damage in unirradiated cells either in the vicinity of irradiated cells or exposed to medium from irradiated cells. This study aimed to further characterize the poorly understood mitochondrial response to both direct irradiation and bystander factor(s) in human keratinocytes (HPV-G) and Chinese hamster ovarian cells (CHO-K1). Oxygen consumption rates were determined during periods of state 4, state 3 and uncoupled respiration. Mitochondrial mass was determined using MitoTracker FM. CHO-K1 cells showed significantly reduced oxygen consumption rates 4 h after exposure to 5 Gy direct radiation and irradiated cell conditioned medium (ICCM) and an apparent recovery 12-24 h later. The apparent recovery was likely due to the substantial increase in mitochondrial mass observed in these cells as soon as 4 h after exposure. HPV-G cells, on the other hand, showed a sustained increase in oxygen consumption rates after ICCM exposure and a transient increase 4 h after exposure to 5 Gy direct radiation. A significant increase in mitochondrial mass per HPV-G cell was observed after exposure to both direct radiation and ICCM. These findings are indicative of a stress response to mitochondrial dysfunction that increases the number of mitochondria per cell.


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