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dc.contributor.authorMaguire, Paula*
dc.contributor.authorMothersill, Carmel*
dc.contributor.authorMcClean, Brendan*
dc.contributor.authorSeymour, Colin*
dc.contributor.authorLyng, Fiona M*
dc.date.accessioned2017-05-29T14:32:33Z
dc.date.available2017-05-29T14:32:33Z
dc.date.issued2007-04
dc.identifier.citationModulation of radiation responses by pre-exposure to irradiated cell conditioned medium. 2007, 167 (4):485-92 Radiat. Res.en
dc.identifier.issn0033-7587
dc.identifier.pmid17388689
dc.identifier.doi10.1667/RR0159.1
dc.identifier.urihttp://hdl.handle.net/10147/621394
dc.description.abstractThe aim of this study was to investigate whether exposure of HPV-G cells to irradiated cell conditioned medium (ICCM) could induce an adaptive response if the cells were subsequently challenged with a higher ICCM dose. Clonogenic survival and major steps in the cascade leading to apoptosis, such as calcium influx and loss of mitochondrial membrane potential, were examined to determine whether these events could be modified by giving a priming dose of ICCM before the challenge dose. Clonogenic survival data indicated an ICCM-induced adaptive response in HPV-G cells "primed" with 5 mGy or 0.5 Gy ICCM for 24 h and then exposed to 0.5 Gy or 5 Gy ICCM. Reactive oxygen species (ROS) were found to be involved in the bystander-induced cell death. Calcium fluxes varied in magnitude across the exposed cell population, and a significant number of the primed HPV-G cells did not respond to the challenge ICCM dose. No significant loss of mitochondrial membrane potential was observed when HPV-G cells were exposed to 0.5 Gy ICCM for 24 h followed by exposure to 5 Gy ICCM for 6 h. Exposure of HPV-G cells to 5 mGy ICCM for 24 h followed by exposure to 0.5 Gy ICCM for 18 h caused a significant increase in mitochondrial mass and a change in mitochondrial location, events associated with the perpetuation of genomic instability. This study has shown that a priming dose of ICCM has the ability to induce an adaptive response in HPV-G cells subsequently exposed to a challenge dose of ICCM.
dc.language.isoenen
dc.publisherRadiation Research Societyen
dc.rightsArchived with thanks to Radiation researchen
dc.subjectHUMAN PAPILLOMA VIRUSen
dc.subjectNEOPLASMSen
dc.subjectCANCERen
dc.subjectRADIOTHERAPYen
dc.subject.meshAdaptation, Physiological
dc.subject.meshCell Line
dc.subject.meshCell Survival
dc.subject.meshCulture Media, Conditioned
dc.subject.meshDNA
dc.subject.meshDNA Damage
dc.subject.meshDose-Response Relationship, Radiation
dc.subject.meshHumans
dc.subject.meshKeratinocytes
dc.subject.meshRadiation Dosage
dc.subject.meshRadiation Tolerance
dc.titleModulation of Radiation responses by pre-exposure to irradiated Cell conditioned medium.en
dc.typeArticleen
dc.identifier.journalRadiation researchen
dc.description.fundingNo fundingen
dc.description.provinceLeinsteren
dc.description.peer-reviewpeer-reviewen
refterms.dateFOA2018-08-27T21:25:48Z
html.description.abstractThe aim of this study was to investigate whether exposure of HPV-G cells to irradiated cell conditioned medium (ICCM) could induce an adaptive response if the cells were subsequently challenged with a higher ICCM dose. Clonogenic survival and major steps in the cascade leading to apoptosis, such as calcium influx and loss of mitochondrial membrane potential, were examined to determine whether these events could be modified by giving a priming dose of ICCM before the challenge dose. Clonogenic survival data indicated an ICCM-induced adaptive response in HPV-G cells "primed" with 5 mGy or 0.5 Gy ICCM for 24 h and then exposed to 0.5 Gy or 5 Gy ICCM. Reactive oxygen species (ROS) were found to be involved in the bystander-induced cell death. Calcium fluxes varied in magnitude across the exposed cell population, and a significant number of the primed HPV-G cells did not respond to the challenge ICCM dose. No significant loss of mitochondrial membrane potential was observed when HPV-G cells were exposed to 0.5 Gy ICCM for 24 h followed by exposure to 5 Gy ICCM for 6 h. Exposure of HPV-G cells to 5 mGy ICCM for 24 h followed by exposure to 0.5 Gy ICCM for 18 h caused a significant increase in mitochondrial mass and a change in mitochondrial location, events associated with the perpetuation of genomic instability. This study has shown that a priming dose of ICCM has the ability to induce an adaptive response in HPV-G cells subsequently exposed to a challenge dose of ICCM.


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