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    A complementary role of multiparameter flow cytometry and high-throughput sequencing for minimal residual disease detection in chronic lymphocytic leukemia: an European Research Initiative on CLL study.

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    Name:
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    Authors
    Rawstron, A C
    Fazi, C
    Agathangelidis, A
    Villamor, N
    Letestu, R
    Nomdedeu, J
    Palacio, C
    Stehlikova, O
    Kreuzer, K-A
    Liptrot, S
    O'Brien, D
    de Tute, R M
    Marinov, I
    Hauwel, M
    Spacek, M
    Dobber, J
    Kater, A P
    Gambell, P
    Soosapilla, A
    Lozanski, G
    Brachtl, G
    Lin, K
    Boysen, J
    Hanson, C
    Jorgensen, J L
    Stetler-Stevenson, M
    Yuan, C
    Broome, H E
    Rassenti, L
    Craig, F
    Delgado, J
    Moreno, C
    Bosch, F
    Egle, A
    Doubek, M
    Pospisilova, S
    Mulligan, S
    Westerman, D
    Sanders, C M
    Emerson, R
    Robins, H S
    Kirsch, I
    Shanafelt, T
    Pettitt, A
    Kipps, T J
    Wierda, W G
    Cymbalista, F
    Hallek, M
    Hillmen, P
    Montserrat, E
    Ghia, P
    Show allShow less
    Issue Date
    2016-04
    Keywords
    LEUKAEMIA
    MeSH
    Adolescent
    Adult
    Antigens, CD
    Combined Modality Therapy
    Europe
    Female
    Flow Cytometry
    Follow-Up Studies
    High-Throughput Nucleotide Sequencing
    Humans
    Immunophenotyping
    Leukemia, Lymphocytic, Chronic, B-Cell
    Male
    Neoplasm Staging
    Neoplasm, Residual
    Prognosis
    Young Adult
    Show allShow less
    
    Metadata
    Show full item record
    Citation
    A complementary role of multiparameter flow cytometry and high-throughput sequencing for minimal residual disease detection in chronic lymphocytic leukemia: an European Research Initiative on CLL study. 2016, 30 (4):929-36 Leukemia
    Journal
    Leukemia
    URI
    http://hdl.handle.net/10147/621126
    DOI
    10.1038/leu.2015.313
    PubMed ID
    26639181
    Abstract
    In chronic lymphocytic leukemia (CLL) the level of minimal residual disease (MRD) after therapy is an independent predictor of outcome. Given the increasing number of new agents being explored for CLL therapy, using MRD as a surrogate could greatly reduce the time necessary to assess their efficacy. In this European Research Initiative on CLL (ERIC) project we have identified and validated a flow-cytometric approach to reliably quantitate CLL cells to the level of 0.0010% (10(-5)). The assay comprises a core panel of six markers (i.e. CD19, CD20, CD5, CD43, CD79b and CD81) with a component specification independent of instrument and reagents, which can be locally re-validated using normal peripheral blood. This method is directly comparable to previous ERIC-designed assays and also provides a backbone for investigation of new markers. A parallel analysis of high-throughput sequencing using the ClonoSEQ assay showed good concordance with flow cytometry results at the 0.010% (10(-4)) level, the MRD threshold defined in the 2008 International Workshop on CLL guidelines, but it also provides good linearity to a detection limit of 1 in a million (10(-6)). The combination of both technologies would permit a highly sensitive approach to MRD detection while providing a reproducible and broadly accessible method to quantify residual disease and optimize treatment in CLL.
    Item Type
    Article
    Language
    en
    ISSN
    1476-5551
    ae974a485f413a2113503eed53cd6c53
    10.1038/leu.2015.313
    Scopus Count
    Collections
    St. James's Hospital

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