Gene expression analysis in prostate cancer: the importance of the endogenous control.
Madden, Stephen F
Lynch, Thomas H
Perry, Antoinette S
AffiliationProstate Molecular Oncology, Academic Unit of Clinical and Molecular Oncology, Institute of Molecular Medicine, Trinity College Dublin, Ireland. email@example.com
Gene Expression Regulation, Neoplastic
Glutathione S-Transferase pi
Hypoxia-Inducible Factor 1, alpha Subunit
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
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CitationVajda A et al. Gene expression analysis in prostate cancer: the importance of the endogenous control. Prostate. 2013, 73 (4):382-90
AbstractAberrant gene expression is a hallmark of cancer. Quantitative reverse-transcription PCR (qRT-PCR) is the gold-standard for quantifying gene expression, and commonly employs a house-keeping gene (HKG) as an endogenous control to normalize results; the choice of which is critical for accurate data interpretation. Many factors, including sample type, pathological state, and oxygen levels influence gene expression including putative HKGs. The aim of this study was to determine the suitability of commonly used HKGs for qRT-PCR in prostate cancer.