• Login
    View Item 
    •   Home
    • Irish Health Organisations
    • Publications
    • View Item
    •   Home
    • Irish Health Organisations
    • Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Map of Submissions

    Home Page
    UlsterN
    5118
    UlsterS
    5118
    Connacht
    1710
    Munster
    58
    Leinster
    467

    Browse

    All of Lenus, The Irish Health RepositoryCommunitiesTitleAuthorsDate publishedSubjectsThis CollectionTitleAuthorsDate publishedSubjects

    My Account

    LoginRegister

    About

    About LenusDirectory of Open Access JournalsOpen Access Publishing GuideNational Health Library & Knowledge ServiceGuide to Publishers' PoliciesFAQsTerms and ConditionsVision StatementORCID Unique identifiers for ResearchersHSE position statement on Open AccessNational Open Research Forum (NORF)Zenodo (European Open Research repository)

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Genome sequencing and comparative analysis of three Chlamydia pecorum strains associated with different pathogenic outcomes

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    1471-2164-15-23.xml
    Size:
    118.0Kb
    Format:
    XML
    Download
    Thumbnail
    Name:
    1471-2164-15-23.pdf
    Size:
    2.261Mb
    Format:
    PDF
    Download
    Thumbnail
    Name:
    1471-2164-15-23-S2.PDF
    Size:
    438.2Kb
    Format:
    PDF
    Download
    Thumbnail
    Name:
    1471-2164-15-23-S1.DOC
    Size:
    146Kb
    Format:
    Microsoft Word
    Download
    View more filesView fewer files
    Authors
    Sait, Michelle
    Livingstone, Morag
    Clark, Ewan M
    Wheelhouse, Nick
    Spalding, Lucy
    Markey, Bryan
    Magnino, Simone
    Lainson, Frederick A
    Myers, Garry SA
    Longbottom, David
    Issue Date
    2014-01-14
    Keywords
    GENETICS
    SEXUALLY TRANSMITTED INFECTION
    
    Metadata
    Show full item record
    Citation
    BMC Genomics. 2014 Jan 14;15(1):23
    URI
    http://dx.doi.org/10.1186/1471-2164-15-23
    http://hdl.handle.net/10147/315444
    Abstract
    Abstract Background Chlamydia pecorum is the causative agent of a number of acute diseases, but most often causes persistent, subclinical infection in ruminants, swine and birds. In this study, the genome sequences of three C. pecorum strains isolated from the faeces of a sheep with inapparent enteric infection (strain W73), from the synovial fluid of a sheep with polyarthritis (strain P787) and from a cervical swab taken from a cow with metritis (strain PV3056/3) were determined using Illumina/Solexa and Roche 454 genome sequencing. Results Gene order and synteny was almost identical between C. pecorum strains and C. psittaci. Differences between C. pecorum and other chlamydiae occurred at a number of loci, including the plasticity zone, which contained a MAC/perforin domain protein, two copies of a >3400 amino acid putative cytotoxin gene and four (PV3056/3) or five (P787 and W73) genes encoding phospholipase D. Chlamydia pecorum contains an almost intact tryptophan biosynthesis operon encoding trpABCDFR and has the ability to sequester kynurenine from its host, however it lacks the genes folA, folKP and folB required for folate metabolism found in other chlamydiae. A total of 15 polymorphic membrane proteins were identified, belonging to six pmp families. Strains possess an intact type III secretion system composed of 18 structural genes and accessory proteins, however a number of putative inc effector proteins widely distributed in chlamydiae are absent from C. pecorum. Two genes encoding the hypothetical protein ORF663 and IncA contain variable numbers of repeat sequences that could be associated with persistence of infection. Conclusions Genome sequencing of three C. pecorum strains, originating from animals with different disease manifestations, has identified differences in ORF663 and pseudogene content between strains and has identified genes and metabolic traits that may influence intracellular survival, pathogenicity and evasion of the host immune system.
    Item Type
    Article
    Language
    en
    Collections
    Publications

    entitlement

     
    Health Library Ireland | Health Service Executive | Jervis House, Jervis Street | Republic of Ireland | Eircode: D01 W596
    lenus@hse.ie | Tel: +353-1-7786275
    DSpace software copyright © 2002-2017  DuraSpace
    Contact Us | Disclaimer
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.