Emergence of hospital- and community-associated panton-valentine leukocidin-positive methicillin-resistant Staphylococcus aureus genotype ST772-MRSA-V in Ireland and detailed investigation of an ST772-MRSA-V cluster in a neonatal intensive care unit.
Authors
Brennan, Gráinne IShore, Anna C
Corcoran, Suzanne
Tecklenborg, Sarah
Coleman, David C
O'Connell, Brian
Affiliation
National MRSA Reference Laboratory, St. James’s Hospital, Dublin, Ireland. gbrennan@stjames.ieIssue Date
2012-03MeSH
AdultAged, 80 and over
Bacterial Toxins
Child
Community-Acquired Infections
Cross Infection
Drug Resistance, Multiple, Bacterial
Exotoxins
Genotype
Humans
Infant
Infant, Newborn
Intensive Care, Neonatal
Ireland
Leukocidins
Methicillin-Resistant Staphylococcus aureus
Microarray Analysis
Molecular Epidemiology
Molecular Typing
Staphylococcal Infections
Virulence Factors
Young Adult
Metadata
Show full item recordCitation
Emergence of hospital- and community-associated panton-valentine leukocidin-positive methicillin-resistant Staphylococcus aureus genotype ST772-MRSA-V in Ireland and detailed investigation of an ST772-MRSA-V cluster in a neonatal intensive care unit. 2012, 50 (3):841-7 J. Clin. Microbiol.Journal
Journal of clinical microbiologyDOI
10.1128/JCM.06354-11PubMed ID
22189119Abstract
Sequence type 22 (ST22) methicillin-resistant Staphylococcus aureus (MRSA) harboring staphylococcal cassette chromosome mec (SCCmec) IV (ST22-MRSA-IV) has predominated in Irish hospitals since the late 1990s. Six distinct clones of community-associated MRSA (CA-MRSA) have also been identified in Ireland. A new strain of CA-MRSA, ST772-MRSA-V, has recently emerged and become widespread in India and has spread into hospitals. In the present study, highly similar MRSA isolates were recovered from seven colonized neonates in a neonatal intensive care unit (NICU) in a maternity hospital in Ireland during 2010 and 2011, two colonized NICU staff, one of their colonized children, and a NICU environmental site. The isolates exhibited multiantibiotic resistance, spa type t657, and were assigned to ST772-MRSA-V by DNA microarray profiling. All isolates encoded resistance to macrolides [msr(A) and mpb(BM)] and aminoglycosides (aacA-aphD and aphA3) and harbored the Panton-Valentine leukocidin toxin genes (lukF-PV and lukS-PV), enterotoxin genes (sea, sec, sel, and egc), and one of the immune evasion complex genes (scn). One of the NICU staff colonized by ST772-MRSA-V was identified as the probable index case, based on recent travel to India. Seven additional hospital and CA-ST772-MRSA-V isolates recovered from skin and soft tissue infections in Ireland between 2009 and 2011 exhibiting highly similar phenotypic and genotypic characteristics to the NICU isolates were also identified. The clinical details of four of these patients revealed connections with India through ethnic background or travel. Our study indicates that hospital-acquired and CA-ST772-MRSA-V is currently emerging in Ireland and may have been imported from India on several occasions.Item Type
ArticleLanguage
enISSN
1098-660Xae974a485f413a2113503eed53cd6c53
10.1128/JCM.06354-11