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    Transcript profiling reveals rewiring of iron assimilation gene expression in Candida albicans and C. dubliniensis.

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    Authors
    Moran, Gary P
    Affiliation
    Division of Oral Biosciences, Dublin Dental School and Hospital, Trinity College Dublin, University of Dublin, Ireland. gpmoran@dental.tcd.ie
    Issue Date
    2012-12
    MeSH
    Candida
    Candida albicans
    Fungal Proteins
    Gene Expression Profiling
    Gene Expression Regulation, Fungal
    Hyphae
    Iron
    Repressor Proteins
    Transcription Factors
    Transcriptome
    
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    Citation
    Transcript profiling reveals rewiring of iron assimilation gene expression in Candida albicans and C. dubliniensis. 2012, 12 (8):918-23 FEMS Yeast Res.
    Publisher
    FEMS yeast research
    Journal
    FEMS yeast research
    URI
    http://hdl.handle.net/10147/292522
    DOI
    10.1111/j.1567-1364.2012.00841.x
    PubMed ID
    22888912
    Abstract
    Hyphal growth is repressed in Candida albicans and Candida dubliniensis by the transcription factor Nrg1. Transcript profiling of a C. dubliniensis NRG1 mutant identified a common group of 28 NRG1-repressed genes in both species, including the hypha-specific genes HWP1, ECE1 and the regulator of cell elongation UME6. Unexpectedly, C. dubliniensis NRG1 was required for wild-type levels of expression of 10 genes required for iron uptake including seven ferric reductases, SIT1, FTR1 and RBT5. However, at alkaline pH and during filamentous growth in 10% serum, most of these genes were highly induced in C. dubliniensis. Conversely, RBT5, PGA10, FRE10 and FRP1 did not exhibit induction during hyphal growth when NRG1 is downregulated, indicating that in C. dubliniensis NRG1 is also required for optimal expression of these genes in alkaline environments. In iron-depleted medium at pH 4.5, reduced growth of the NRG1 mutant relative to wild type was observed; however, growth was restored to wild-type levels or greater at pH 6.5, indicating that alkaline induction of iron assimilation gene expression could rescue this phenotype. These data indicate that transcriptional control of iron assimilation and pseudohypha formation has been separated in C. albicans, perhaps promoting growth in a wider range of niches.
    Item Type
    Article
    Language
    en
    ISSN
    1567-1364
    ae974a485f413a2113503eed53cd6c53
    10.1111/j.1567-1364.2012.00841.x
    Scopus Count
    Collections
    Dublin Dental University Hospital

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