• Login
    View Item 
    •   Home
    • Hospital Research
    • Leinster
    • Dublin Dental University Hospital
    • View Item
    •   Home
    • Hospital Research
    • Leinster
    • Dublin Dental University Hospital
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Map of Submissions

    Home Page
    UlsterN
    4379
    UlsterS
    4379
    Connacht
    1416
    Munster
    63
    Leinster
    427

    Browse

    All of Lenus, The Irish Health RepositoryCommunitiesTitleAuthorsDate publishedSubjectsThis CollectionTitleAuthorsDate publishedSubjects

    My Account

    LoginRegister

    About

    About LenusDirectory of Open Access JournalsOpen Access Publishing GuideHSE Library Guide to Publishers' PoliciesFAQsTerms and ConditionsVision StatementORCID Unique identifiers for ResearchersHSE position statement on Open AccessNational Open Research Forum (NORF)Zenodo (European Open Research repository)

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    DNA microarray profiling of a diverse collection of nosocomial methicillin-resistant staphylococcus aureus isolates assigns the majority to the correct sequence type and staphylococcal cassette chromosome mec (SCCmec) type and results in the subsequent identification and characterization of novel SCCmec-SCCM1 composite islands.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    zac5340.pdf
    Size:
    746.5Kb
    Format:
    PDF
    Download
    Authors
    Shore, Anna C
    Brennan, Orla M
    Deasy, Emily C
    Rossney, Angela S
    Kinnevey, Peter M
    Ehricht, Ralf
    Monecke, Stefan
    Coleman, David C
    Affiliation
    Microbiology Research Unit, Dublin Dental University Hospital, University of Dublin, Trinity College, Dublin, Ireland.
    Issue Date
    2012-10
    MeSH
    Bacterial Proteins
    Fusidic Acid
    Methicillin-Resistant Staphylococcus aureus
    Multilocus Sequence Typing
    Oligonucleotide Array Sequence Analysis
    Trimethoprim
    
    Metadata
    Show full item record
    Citation
    DNA microarray profiling of a diverse collection of nosocomial methicillin-resistant staphylococcus aureus isolates assigns the majority to the correct sequence type and staphylococcal cassette chromosome mec (SCCmec) type and results in the subsequent identification and characterization of novel SCCmec-SCCM1 composite islands. 2012, 56 (10):5340-55 Antimicrob. Agents Chemother.
    Publisher
    Antimicrobial agents and chemotherapy
    Journal
    Antimicrobial agents and chemotherapy
    URI
    http://hdl.handle.net/10147/292509
    DOI
    10.1128/AAC.01247-12
    PubMed ID
    22869569
    Abstract
    One hundred seventy-five isolates representative of methicillin-resistant Staphylococcus aureus (MRSA) clones that predominated in Irish hospitals between 1971 and 2004 and that previously underwent multilocus sequence typing (MLST) and staphylococcal cassette chromosome mec (SCCmec) typing were characterized by spa typing (175 isolates) and DNA microarray profiling (107 isolates). The isolates belonged to 26 sequence type (ST)-SCCmec types and subtypes and 35 spa types. The array assigned all isolates to the correct MLST clonal complex (CC), and 94% (100/107) were assigned an ST, with 98% (98/100) correlating with MLST. The array assigned all isolates to the correct SCCmec type, but subtyping of only some SCCmec elements was possible. Additional SCCmec/SCC genes or DNA sequence variation not detected by SCCmec typing was detected by array profiling, including the SCC-fusidic acid resistance determinant Q6GD50/fusC. Novel SCCmec/SCC composite islands (CIs) were detected among CC8 isolates and comprised SCCmec IIA-IIE, IVE, IVF, or IVg and a ccrAB4-SCC element with 99% DNA sequence identity to SCC(M1) from ST8/t024-MRSA, SCCmec VIII, and SCC-CI in Staphylococcus epidermidis. The array showed that the majority of isolates harbored one or more superantigen (94%; 100/107) and immune evasion cluster (91%; 97/107) genes. Apart from fusidic acid and trimethoprim resistance, the correlation between isolate antimicrobial resistance phenotype and the presence of specific resistance genes was ≥97%. Array profiling allowed high-throughput, accurate assignment of MRSA to CCs/STs and SCCmec types and provided further evidence of the diversity of SCCmec/SCC. In most cases, array profiling can accurately predict the resistance phenotype of an isolate.
    Item Type
    Article
    Language
    en
    ISSN
    1098-6596
    ae974a485f413a2113503eed53cd6c53
    10.1128/AAC.01247-12
    Scopus Count
    Collections
    Dublin Dental University Hospital

    entitlement

    Related articles

    • Detection of staphylococcal cassette chromosome mec-associated DNA segments in multiresistant methicillin-susceptible Staphylococcus aureus (MSSA) and identification of Staphylococcus epidermidis ccrAB4 in both methicillin-resistant S. aureus and MSSA.
    • Authors: Shore AC, Rossney AS, O'Connell B, Herra CM, Sullivan DJ, Humphreys H, Coleman DC
    • Issue date: 2008 Dec
    • Extensive genetic diversity identified among sporadic methicillin-resistant Staphylococcus aureus isolates recovered in Irish hospitals between 2000 and 2012.
    • Authors: Kinnevey PM, Shore AC, Brennan GI, Sullivan DJ, Ehricht R, Monecke S, Coleman DC
    • Issue date: 2014
    • Emergence of sequence type 779 methicillin-resistant Staphylococcus aureus harboring a novel pseudo staphylococcal cassette chromosome mec (SCCmec)-SCC-SCCCRISPR composite element in Irish hospitals.
    • Authors: Kinnevey PM, Shore AC, Brennan GI, Sullivan DJ, Ehricht R, Monecke S, Slickers P, Coleman DC
    • Issue date: 2013 Jan
    • Comparative Genotypes, Staphylococcal Cassette Chromosome mec (SCCmec) Genes and Antimicrobial Resistance amongst Staphylococcus epidermidis and Staphylococcus haemolyticus Isolates from Infections in Humans and Companion Animals.
    • Authors: McManus BA, Coleman DC, Deasy EC, Brennan GI, O' Connell B, Monecke S, Ehricht R, Leggett B, Leonard N, Shore AC
    • Issue date: 2015
    • Genotyping of community-associated methicillin resistant Staphylococcus aureus (CA-MRSA) in a tertiary care centre in Mysore, South India: ST2371-SCCmec IV emerges as the major clone.
    • Authors: Rajan V, Schoenfelder SM, Ziebuhr W, Gopal S
    • Issue date: 2015 Aug
    HSE Library | Health Service Executive | Dr Steevens’ Hospital, Dublin 8 | Republic of Ireland | Eircode: D08 W2A8
    lenus@hse.ie
    DSpace software copyright © 2002-2017  DuraSpace
    Contact Us | Disclaimer
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.