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dc.contributor.authorHiggins, J
dc.contributor.authorPinjon, E
dc.contributor.authorOltean, H N
dc.contributor.authorWhite, T C
dc.contributor.authorKelly, S L
dc.contributor.authorMartel, C M
dc.contributor.authorSullivan, D J
dc.contributor.authorColeman, D C
dc.contributor.authorMoran, G P
dc.date.accessioned2013-05-21T11:34:51Z
dc.date.available2013-05-21T11:34:51Z
dc.date.issued2012-01
dc.identifier.citationTriclosan antagonizes fluconazole activity against Candida albicans. 2012, 91 (1):65-70 J. Dent. Res.en_GB
dc.identifier.issn1544-0591
dc.identifier.pmid21972257
dc.identifier.doi10.1177/0022034511425046
dc.identifier.urihttp://hdl.handle.net/10147/292497
dc.description.abstractTriclosan is a broad-spectrum antimicrobial compound commonly used in oral hygiene products. Investigation of its activity against Candida albicans showed that triclosan was fungicidal at concentrations of 16 mg/L. However, at subinhibitory concentrations (0.5-2 mg/L), triclosan antagonized the activity of fluconazole. Although triclosan induced CDR1 expression in C. albicans, antagonism was still observed in cdr1Δ and cdr2Δ strains. Triclosan did not affect fluconazole uptake or alter total membrane sterol content, but did induce the expression of FAS1 and FAS2, indicating that its mode of action may involve inhibition of fatty acid synthesis, as it does in prokaryotes. However, FAS2 mutants did not exhibit increased susceptibility to triclosan, and overexpression of both FAS1 and FAS2 alleles did not alter triclosan susceptibility. Unexpectedly, the antagonistic effect was specific for C. albicans under hypha-inducing conditions and was absent in the non-filamentous efg1Δ strain. This antagonism may be due to the membranotropic activity of triclosan and the unique composition of hyphal membranes.
dc.language.isoenen
dc.publisherJournal of dental researchen_GB
dc.rightsArchived with thanks to Journal of dental researchen_GB
dc.subject.meshAntifungal Agents
dc.subject.meshCandida albicans
dc.subject.meshDrug Antagonism
dc.subject.meshFatty Acids
dc.subject.meshFluconazole
dc.subject.meshFungal Proteins
dc.subject.meshHyphae
dc.subject.meshMembrane Transport Proteins
dc.subject.meshMicrobial Sensitivity Tests
dc.subject.meshSpecies Specificity
dc.subject.meshTriclosan
dc.titleTriclosan antagonizes fluconazole activity against Candida albicans.en_GB
dc.typeArticleen
dc.contributor.departmentMicrobiology Research Unit, Division of Oral Biosciences, Dublin Dental University Hospital, University of Dublin, Trinity College Dublin, Lincoln Place, Dublin 2, Ireland.en_GB
dc.identifier.journalJournal of dental researchen_GB
dc.description.provinceLeinsteren
html.description.abstractTriclosan is a broad-spectrum antimicrobial compound commonly used in oral hygiene products. Investigation of its activity against Candida albicans showed that triclosan was fungicidal at concentrations of 16 mg/L. However, at subinhibitory concentrations (0.5-2 mg/L), triclosan antagonized the activity of fluconazole. Although triclosan induced CDR1 expression in C. albicans, antagonism was still observed in cdr1Δ and cdr2Δ strains. Triclosan did not affect fluconazole uptake or alter total membrane sterol content, but did induce the expression of FAS1 and FAS2, indicating that its mode of action may involve inhibition of fatty acid synthesis, as it does in prokaryotes. However, FAS2 mutants did not exhibit increased susceptibility to triclosan, and overexpression of both FAS1 and FAS2 alleles did not alter triclosan susceptibility. Unexpectedly, the antagonistic effect was specific for C. albicans under hypha-inducing conditions and was absent in the non-filamentous efg1Δ strain. This antagonism may be due to the membranotropic activity of triclosan and the unique composition of hyphal membranes.


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