Triclosan antagonizes fluconazole activity against Candida albicans.
Authors
Higgins, JPinjon, E
Oltean, H N
White, T C
Kelly, S L
Martel, C M
Sullivan, D J
Coleman, D C
Moran, G P
Affiliation
Microbiology Research Unit, Division of Oral Biosciences, Dublin Dental University Hospital, University of Dublin, Trinity College Dublin, Lincoln Place, Dublin 2, Ireland.Issue Date
2012-01MeSH
Antifungal AgentsCandida albicans
Drug Antagonism
Fatty Acids
Fluconazole
Fungal Proteins
Hyphae
Membrane Transport Proteins
Microbial Sensitivity Tests
Species Specificity
Triclosan
Metadata
Show full item recordCitation
Triclosan antagonizes fluconazole activity against Candida albicans. 2012, 91 (1):65-70 J. Dent. Res.Publisher
Journal of dental researchJournal
Journal of dental researchDOI
10.1177/0022034511425046PubMed ID
21972257Abstract
Triclosan is a broad-spectrum antimicrobial compound commonly used in oral hygiene products. Investigation of its activity against Candida albicans showed that triclosan was fungicidal at concentrations of 16 mg/L. However, at subinhibitory concentrations (0.5-2 mg/L), triclosan antagonized the activity of fluconazole. Although triclosan induced CDR1 expression in C. albicans, antagonism was still observed in cdr1Δ and cdr2Δ strains. Triclosan did not affect fluconazole uptake or alter total membrane sterol content, but did induce the expression of FAS1 and FAS2, indicating that its mode of action may involve inhibition of fatty acid synthesis, as it does in prokaryotes. However, FAS2 mutants did not exhibit increased susceptibility to triclosan, and overexpression of both FAS1 and FAS2 alleles did not alter triclosan susceptibility. Unexpectedly, the antagonistic effect was specific for C. albicans under hypha-inducing conditions and was absent in the non-filamentous efg1Δ strain. This antagonism may be due to the membranotropic activity of triclosan and the unique composition of hyphal membranes.Item Type
ArticleLanguage
enISSN
1544-0591ae974a485f413a2113503eed53cd6c53
10.1177/0022034511425046
Scopus Count
Collections
Related articles
- The fungicide fludioxonil antagonizes fluconazole activity in the human fungal pathogen Candida albicans.
- Authors: Buschart A, Burakowska A, Bilitewski U
- Issue date: 2012 Dec
- Antagonism of azole activity against Candida albicans following induction of multidrug resistance genes by selected antimicrobial agents.
- Authors: Henry KW, Cruz MC, Katiyar SK, Edlind TD
- Issue date: 1999 Aug
- Effects of cetylpyridinium chloride resistance and treatment on fluconazole activity versus Candida albicans.
- Authors: Edlind MP, Smith WL, Edlind TD
- Issue date: 2005 Feb
- A new triazole, voriconazole (UK-109,496), blocks sterol biosynthesis in Candida albicans and Candida krusei.
- Authors: Sanati H, Belanger P, Fratti R, Ghannoum M
- Issue date: 1997 Nov
- Potent drugs that attenuate anti-Candida albicans activity of fluconazole and their possible mechanisms of action.
- Authors: Urai M, Kaneko Y, Niki M, Inoue M, Tanabe K, Umeyama T, Fukazawa H, Ohno H, Miyazaki Y
- Issue date: 2014 Oct