Molecular-based mycobacterial identification in a clinical laboratory setting: a comparison of two methods.
AffiliationDepartment of Medical Microbiology, Cork University Hospital, Cork, Ireland.
Mycobacterium Infections, Nontuberculous
MetadataShow full item record
CitationMolecular-based mycobacterial identification in a clinical laboratory setting: a comparison of two methods. 2012, 69 (4):164-8 Br. J. Biomed. Sci.
JournalBritish journal of biomedical science
AbstractMany mycobacterial species are pathogenic to humans, with infection occurring worldwide. Infection with Mycobacterium tuberculosis is a well-described global phenomenon, but other mycobacterial species are increasingly shown to be the cause of both pulmonary and extrapulmonary infection and are managed differently from M. tuberculosis infection. Rapid and accurate differentiation of mycobacterial species is, therefore, critical to guide timely and appropriate therapeutic and public health management. This study evaluates two commercially available DNA strip assays, the Genotype Common Mycobacteria (CM) assay (Hain Lifescience, Nehren, Germany) and the Speed-oligo Mycobacteria assay (Vircell, Spain) for their usefulness in a clinical laboratory setting. Both assays were evaluated on 71 clinical mycobacterial isolates, previously identified using Gen-Probe AccuProbe and through a UK mycobacteriology reference laboratory, as well as 29 non-mycobacterial isolates. Concordant results were obtained for 98% of isolates using both assays. The sensitivity was 97% (95% confidence interval [CI]: 93.3-100%) for the CM assay and 98.6% (95% CI: 95.9-100%) for the Speed-oligo assay. Overall, both assays proved to be useful tools for rapid and sensitive mycobacterial species identification, although interpretation of results was easier with the CM assay. Finally, results were available within one day, compared to current identification times which range between seven days and four weeks.
- [Evaluation of the distribution of non-tuberculous mycobacteria strains isolated in National Tuberculosis Reference Laboratory in 2009-2010, Turkey].
- Authors: Albayrak N, Simşek H, Sezen F, Arslantürk A, Tarhan G, Ceyhan I
- Issue date: 2012 Oct
- Multi-centre evaluation of the speed-oligo Mycobacteria assay for differentiation of Mycobacterium spp. in clinical isolates.
- Authors: Hofmann-Thiel S, Turaev L, Alnour T, Drath L, Müllerova M, Hoffmann H
- Issue date: 2011 Dec 19
- Evaluation of the GenoType Mycobacteria Direct assay for detection of Mycobacterium tuberculosis complex and four atypical mycobacterial species in clinical samples.
- Authors: Franco-Alvarez de Luna F, Ruiz P, Gutiérrez J, Casal M
- Issue date: 2006 Aug
- Evaluation of the GenoType Mycobacteria Direct assay for the simultaneous detection of the Mycobacterium tuberculosis complex and four atypical mycobacterial species in smear-positive respiratory specimens.
- Authors: Seagar AL, Prendergast C, Emmanuel FX, Rayner A, Thomson S, Laurenson IF
- Issue date: 2008 May
- Evaluation of a membrane array for detection of Mycobacterium tuberculosis complex and nontuberculous mycobacteria in positive liquid cultures.
- Authors: Lu PL, Lin YC, Yang YC, Jou R, Huang SC, Jenh YS, Huang HH, Chang TC
- Issue date: 2013 Apr