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    Molecular-based mycobacterial identification in a clinical laboratory setting: a comparison of two methods.

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    Authors
    O'Donnell, N
    Corcoran, D
    Lucey, B
    Barrett, A
    Affiliation
    Department of Medical Microbiology, Cork University Hospital, Cork, Ireland.
    Issue Date
    2012
    MeSH
    Genotype
    Humans
    Laboratories, Hospital
    Microbiological Techniques
    Mycobacterium Infections, Nontuberculous
    Nontuberculous Mycobacteria
    
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    Citation
    Molecular-based mycobacterial identification in a clinical laboratory setting: a comparison of two methods. 2012, 69 (4):164-8 Br. J. Biomed. Sci.
    Journal
    British journal of biomedical science
    URI
    http://hdl.handle.net/10147/267992
    PubMed ID
    23304792
    Abstract
    Many mycobacterial species are pathogenic to humans, with infection occurring worldwide. Infection with Mycobacterium tuberculosis is a well-described global phenomenon, but other mycobacterial species are increasingly shown to be the cause of both pulmonary and extrapulmonary infection and are managed differently from M. tuberculosis infection. Rapid and accurate differentiation of mycobacterial species is, therefore, critical to guide timely and appropriate therapeutic and public health management. This study evaluates two commercially available DNA strip assays, the Genotype Common Mycobacteria (CM) assay (Hain Lifescience, Nehren, Germany) and the Speed-oligo Mycobacteria assay (Vircell, Spain) for their usefulness in a clinical laboratory setting. Both assays were evaluated on 71 clinical mycobacterial isolates, previously identified using Gen-Probe AccuProbe and through a UK mycobacteriology reference laboratory, as well as 29 non-mycobacterial isolates. Concordant results were obtained for 98% of isolates using both assays. The sensitivity was 97% (95% confidence interval [CI]: 93.3-100%) for the CM assay and 98.6% (95% CI: 95.9-100%) for the Speed-oligo assay. Overall, both assays proved to be useful tools for rapid and sensitive mycobacterial species identification, although interpretation of results was easier with the CM assay. Finally, results were available within one day, compared to current identification times which range between seven days and four weeks.
    Item Type
    Article
    Language
    en
    ISSN
    0967-4845
    Collections
    Cork University Hospital

    entitlement

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