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dc.contributor.authorShaw, Christopher S
dc.contributor.authorSherlock, Mark
dc.contributor.authorStewart, Paul M
dc.contributor.authorWagenmakers, Anton J M
dc.date.accessioned2012-11-30T16:36:14Z
dc.date.available2012-11-30T16:36:14Z
dc.date.issued2009-05
dc.identifier.citationAdipophilin distribution and colocalization with lipid droplets in skeletal muscle. 2009, 131 (5):575-81 Histochem. Cell Biol.en_GB
dc.identifier.issn1432-119X
dc.identifier.pmid19169702
dc.identifier.doi10.1007/s00418-009-0558-4
dc.identifier.urihttp://hdl.handle.net/10147/254132
dc.description.abstractIntramyocellular lipids (IMCL) are stored as discrete lipid droplets which are associated with a number of proteins. The lipid droplet-associated protein adipophilin (the human orthologue of adipose differentiation-related protein) is ubiquitously expressed and is one of the predominant lipid droplet-proteins in skeletal muscle. The aim of this study was to investigate the subcellular distribution of adipophilin in human muscle fibres and to measure the colocalization of adipophilin with IMCL. Muscle biopsies from six lean male cyclists (BMI 23.4 +/- 0.4, aged 31 +/- 2 years, W (max) 346 +/- 8) were stained for myosin heavy chain type 1, IMCL, adipophilin and mitochondria using immunofluorescence and viewed with widefield and confocal fluorescence microscopy. The present study shows that like IMCL, the adipophilin content is ~twofold greater in type I skeletal muscle fibres and is situated in the areas between the mitochondrial network. Colocalization analysis demonstrated that 61 +/- 2% of IMCL contain adipophilin. Although the majority of adipophilin is contained within IMCL, 36 +/- 4% of adipophilin is not associated with IMCL. In conclusion, this study indicates that the IMCL pool is heterogeneous, as the majority but not all IMCL contain adipophilin.
dc.language.isoenen
dc.publisherHistochemistry and cell biologyen_GB
dc.rightsArchived with thanks to Histochemistry and cell biologyen_GB
dc.subject.meshAdult
dc.subject.meshHumans
dc.subject.meshLipids
dc.subject.meshMale
dc.subject.meshMembrane Proteins
dc.subject.meshMitochondria
dc.subject.meshMuscle Fibers, Skeletal
dc.subject.meshPeptides
dc.titleAdipophilin distribution and colocalization with lipid droplets in skeletal muscle.en_GB
dc.typeArticleen
dc.contributor.departmentExercise Metabolism Research Group, School of Sport and Exercise Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK. c.s.shaw@bham.ac.uken_GB
dc.identifier.journalHistochemistry and cell biologyen_GB
dc.description.provinceLeinsteren
html.description.abstractIntramyocellular lipids (IMCL) are stored as discrete lipid droplets which are associated with a number of proteins. The lipid droplet-associated protein adipophilin (the human orthologue of adipose differentiation-related protein) is ubiquitously expressed and is one of the predominant lipid droplet-proteins in skeletal muscle. The aim of this study was to investigate the subcellular distribution of adipophilin in human muscle fibres and to measure the colocalization of adipophilin with IMCL. Muscle biopsies from six lean male cyclists (BMI 23.4 +/- 0.4, aged 31 +/- 2 years, W (max) 346 +/- 8) were stained for myosin heavy chain type 1, IMCL, adipophilin and mitochondria using immunofluorescence and viewed with widefield and confocal fluorescence microscopy. The present study shows that like IMCL, the adipophilin content is ~twofold greater in type I skeletal muscle fibres and is situated in the areas between the mitochondrial network. Colocalization analysis demonstrated that 61 +/- 2% of IMCL contain adipophilin. Although the majority of adipophilin is contained within IMCL, 36 +/- 4% of adipophilin is not associated with IMCL. In conclusion, this study indicates that the IMCL pool is heterogeneous, as the majority but not all IMCL contain adipophilin.


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