Promoter characterization and genomic organization of the human X11β gene APBA2.
Affiliation
Biochemistry Program, School of Life Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR.Issue Date
2012-02-15MeSH
Base SequenceBinding Sites
Brain
Cadherins
Carrier Proteins
DNA Methylation
Gene Expression Regulation
Genome, Human
Humans
Molecular Sequence Data
Mutagenesis
Nerve Tissue Proteins
Promoter Regions, Genetic
Transcription, Genetic
Metadata
Show full item recordCitation
Promoter characterization and genomic organization of the human X11β gene APBA2. 2012, 23 (3):146-51 NeuroreportJournal
NeuroreportDOI
10.1097/WNR.0b013e32834f1934PubMed ID
22222501Abstract
Overexpression of neuronal adaptor protein X11β has been shown to decrease the production of amyloid-β, a toxic peptide deposited in Alzheimer's disease brains. Therefore, manipulation of the X11β level may represent a potential therapeutic strategy for Alzheimer's disease. As X11β expression can be regulated at the transcription level, we determined the genomic organization and the promoter of the human X11β gene, amyloid β A4 precursor protein-binding family A member 2 (APBA2). By RNA ligase-mediated rapid amplification of cDNA ends, a single APBA2 transcription start site and the complete sequence of exon 1 were identified. The APBA2 promoter was located upstream of exon 1 and was more active in neurons. The core promoter contains several CpG dinucleotides, and was strongly suppressed by DNA methylation. In addition, mutagenesis analysis revealed a putative Pax5-binding site within the promoter. Together, APBA2 contains a potent neuronal promoter whose activity may be regulated by DNA methylation and Pax5.Item Type
ArticleLanguage
enISSN
1473-558Xae974a485f413a2113503eed53cd6c53
10.1097/WNR.0b013e32834f1934
Scopus Count
Collections
Related articles
- Transcriptional regulation of the mouse PNRC2 promoter by the nuclear factor Y (NFY) and E2F1.
- Authors: Zhou D, Masri S, Ye JJ, Chen S
- Issue date: 2005 Nov 21
- The promoter of Alzheimer's disease amyloid A4 precursor gene.
- Authors: Salbaum JM, Weidemann A, Masters CL, Beyreuther K
- Issue date: 1989
- Copy number and sequence variants implicate APBA2 as an autism candidate gene.
- Authors: Babatz TD, Kumar RA, Sudi J, Dobyns WB, Christian SL
- Issue date: 2009 Dec
- Characterization of the human PRIP-1 gene structure and transcriptional regulation.
- Authors: Murakami A, Matsuda M, Nakasima A, Hirata M
- Issue date: 2006 Nov 1
- The neuronal adaptor protein X11beta reduces amyloid beta-protein levels and amyloid plaque formation in the brains of transgenic mice.
- Authors: Lee JH, Lau KF, Perkinton MS, Standen CL, Rogelj B, Falinska A, McLoughlin DM, Miller CC
- Issue date: 2004 Nov 19