• Login
    View Item 
    •   Home
    • Hospital Research
    • Leinster
    • Beaumont Hospital
    • View Item
    •   Home
    • Hospital Research
    • Leinster
    • Beaumont Hospital
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Map of Submissions

    Home Page
    UlsterN
    4378
    UlsterS
    4378
    Connacht
    1408
    Munster
    62
    Leinster
    426

    Browse

    All of Lenus, The Irish Health RepositoryCommunitiesTitleAuthorsDate publishedSubjectsThis CollectionTitleAuthorsDate publishedSubjects

    My Account

    LoginRegister

    About

    About LenusDirectory of Open Access JournalsOpen Access Publishing GuideHSE Library Guide to Publishers' PoliciesFAQsTerms and ConditionsVision StatementORCID Unique identifiers for ResearchersHSE position statement on Open AccessNational Open Research Forum (NORF)Zenodo (European Open Research repository)

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Screening of siRNA nanoparticles for delivery to airway epithelial cells using high-content analysis

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Hibbitts, Alan
    Lieggi, Nora
    McCabe, Olive
    Thomas, Warren
    Barlow, James
    O’Brien, Fiona
    Cryan, Sally-Ann
    Issue Date
    2011-08
    
    Metadata
    Show full item record
    Citation
    Screening of siRNA nanoparticles for delivery to airway epithelial cells using high-content analysis 2011, 2 (8):987 Therapeutic Delivery
    Journal
    Therapeutic Delivery
    URI
    http://hdl.handle.net/10147/240673
    DOI
    10.4155/tde.11.73
    Additional Links
    http://www.future-science.com/doi/abs/10.4155/tde.11.73
    Abstract
    Aims: Delivery of siRNA to the lungs via inhalation offers a unique opportunity to develop a new treatment paradigm for a range of respiratory conditions. However, progress has been greatly hindered by safety and delivery issues. This study developed a high-throughput method for screening novel nanotechnologies for pulmonary siRNA delivery. Methodology: Following physicochemical analysis, the ability of PEI–PEG–siRNA nanoparticles to facilitate siRNA delivery was determined using high-content analysis (HCA) in Calu-3 cells. Results obtained from HCA were validated using confocal microscopy. Finally, cytotoxicity of the PEI–PEG–siRNA particles was analyzed by HCA using the Cellomics® multiparameter cytotoxicity assay. Conclusion: PEI–PEG–siRNA nanoparticles facilitated increased siRNA uptake and luciferase knockdown in Calu-3 cells compared with PEI–siRNA.
    Item Type
    Article
    Language
    en
    ISSN
    2041-5990
    2041-6008
    ae974a485f413a2113503eed53cd6c53
    10.4155/tde.11.73
    Scopus Count
    Collections
    Beaumont Hospital

    entitlement

     
    HSE Library | Health Service Executive | Dr Steevens’ Hospital, Dublin 8 | Republic of Ireland | Eircode: D08 W2A8
    lenus@hse.ie
    DSpace software copyright © 2002-2017  DuraSpace
    Contact Us | Disclaimer
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.