Screening of siRNA nanoparticles for delivery to airway epithelial cells using high-content analysis
Authors
Hibbitts, AlanLieggi, Nora
McCabe, Olive
Thomas, Warren
Barlow, James
O’Brien, Fiona
Cryan, Sally-Ann
Issue Date
2011-08
Metadata
Show full item recordCitation
Screening of siRNA nanoparticles for delivery to airway epithelial cells using high-content analysis 2011, 2 (8):987 Therapeutic DeliveryJournal
Therapeutic DeliveryDOI
10.4155/tde.11.73Additional Links
http://www.future-science.com/doi/abs/10.4155/tde.11.73Abstract
Aims: Delivery of siRNA to the lungs via inhalation offers a unique opportunity to develop a new treatment paradigm for a range of respiratory conditions. However, progress has been greatly hindered by safety and delivery issues. This study developed a high-throughput method for screening novel nanotechnologies for pulmonary siRNA delivery. Methodology: Following physicochemical analysis, the ability of PEI–PEG–siRNA nanoparticles to facilitate siRNA delivery was determined using high-content analysis (HCA) in Calu-3 cells. Results obtained from HCA were validated using confocal microscopy. Finally, cytotoxicity of the PEI–PEG–siRNA particles was analyzed by HCA using the Cellomics® multiparameter cytotoxicity assay. Conclusion: PEI–PEG–siRNA nanoparticles facilitated increased siRNA uptake and luciferase knockdown in Calu-3 cells compared with PEI–siRNA.Item Type
ArticleLanguage
enISSN
2041-59902041-6008
ae974a485f413a2113503eed53cd6c53
10.4155/tde.11.73