• Login
    View Item 
    •   Home
    • Other Irish Health Organisations
    • Publications
    • View Item
    •   Home
    • Other Irish Health Organisations
    • Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Map of Submissions

    Home Page
    UlsterN
    4995
    UlsterS
    4995
    Connacht
    1672
    Munster
    54
    Leinster
    456

    Browse

    All of Lenus, The Irish Health RepositoryCommunitiesTitleAuthorsDate publishedSubjectsThis CollectionTitleAuthorsDate publishedSubjects

    My Account

    LoginRegister

    About

    About LenusDirectory of Open Access JournalsOpen Access Publishing GuideNational Health Library & Knowledge ServiceGuide to Publishers' PoliciesFAQsTerms and ConditionsVision StatementORCID Unique identifiers for ResearchersHSE position statement on Open AccessNational Open Research Forum (NORF)Zenodo (European Open Research repository)

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    MicroRNA-184 inhibits neuroblastoma cell survival through targeting the serine/threonine kinase AKT2

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    1476-4598-9-83.xml
    Size:
    70.33Kb
    Format:
    XML
    Download
    Thumbnail
    Name:
    1476-4598-9-83.pdf
    Size:
    1.828Mb
    Format:
    PDF
    Download
    Thumbnail
    Name:
    1476-4598-9-83-S2.TIFF
    Size:
    2.040Mb
    Format:
    TIFF image
    Download
    Thumbnail
    Name:
    1476-4598-9-83-S4.TIFF
    Size:
    1.811Mb
    Format:
    TIFF image
    Download
    Thumbnail
    Name:
    1476-4598-9-83-S5.TIFF
    Size:
    1.506Mb
    Format:
    TIFF image
    Download
    Thumbnail
    Name:
    1476-4598-9-83-S1.TIFF
    Size:
    1.965Mb
    Format:
    TIFF image
    Download
    Thumbnail
    Name:
    1476-4598-9-83-S3.TIFF
    Size:
    1.500Mb
    Format:
    TIFF image
    Download
    View more filesView fewer files
    Authors
    Foley, Niamh H
    Bray, Isabella M
    Tivnan, Amanda
    Bryan, Kenneth
    Murphy, Derek M
    Buckley, Patrick G
    Ryan, Jacqueline
    O'Meara, Anne
    O'Sullivan, Maureen
    Stallings, Raymond L
    Issue Date
    2010-04-21
    
    Metadata
    Show full item record
    Citation
    Molecular Cancer. 2010 Apr 21;9(1):83
    URI
    http://dx.doi.org/10.1186/1476-4598-9-83
    http://hdl.handle.net/10147/239153
    Abstract
    Abstract Background Neuroblastoma is a paediatric cancer of the sympathetic nervous system. The single most important genetic indicator of poor clinical outcome is amplification of the MYCN transcription factor. One of many down-stream MYCN targets is miR-184, which is either directly or indirectly repressed by this transcription factor, possibly due to its pro-apoptotic effects when ectopically over-expressed in neuroblastoma cells. The purpose of this study was to elucidate the molecular mechanism by which miR-184 conveys pro-apoptotic effects. Results We demonstrate that the knock-down of endogenous miR-184 has the opposite effect of ectopic up-regulation, leading to enhanced neuroblastoma cell numbers. As a mechanism of how miR-184 causes apoptosis when over-expressed, and increased cell numbers when inhibited, we demonstrate direct targeting and degradation of AKT2, a major downstream effector of the phosphatidylinositol 3-kinase (PI3K) pathway, one of the most potent pro-survival pathways in cancer. The pro-apoptotic effects of miR-184 ectopic over-expression in neuroblastoma cell lines is reproduced by siRNA inhibition of AKT2, while a positive effect on cell numbers similar to that obtained by the knock-down of endogenous miR-184 can be achieved by ectopic up-regulation of AKT2. Moreover, co-transfection of miR-184 with an AKT2 expression vector lacking the miR-184 target site in the 3'UTR rescues cells from the pro-apoptotic effects of miR-184. Conclusions MYCN contributes to tumorigenesis, in part, by repressing miR-184, leading to increased levels of AKT2, a direct target of miR-184. Thus, two important genes with positive effects on cell growth and survival, MYCN and AKT2, can be linked into a common genetic pathway through the actions of miR-184. As an inhibitor of AKT2, miR-184 could be of potential benefit in miRNA mediated therapeutics of MYCN amplified neuroblastoma and other forms of cancer.
    Item Type
    Journal Article
    Collections
    Publications

    entitlement

     
    Health Library Ireland | Health Service Executive | Jervis House, Jervis Street | Republic of Ireland | Eircode: D01 W596
    lenus@hse.ie | Tel: +353-1-7786275
    DSpace software copyright © 2002-2017  DuraSpace
    Contact Us | Disclaimer
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.