Show simple item record

dc.contributor.authorZagozdzon, Agnieszka M
dc.contributor.authorO’Leary, Patrick
dc.contributor.authorCallanan, John J
dc.contributor.authorCrown, John
dc.contributor.authorGallagher, William M
dc.contributor.authorZagozdzon, Radoslaw
dc.date.accessioned2012-08-08T15:11:46Z
dc.date.available2012-08-08T15:11:46Z
dc.date.issued2012-05-30
dc.identifier.citationBMC Cancer. 2012 May 30;12(1):209
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2407-12-209
dc.identifier.urihttp://hdl.handle.net/10147/237750
dc.description.abstractAbstractBackgroundNumerous transgenic models have been generated to study breast cancer. However, despite many advantages, traditional transgenic models for breast cancer are also burdened with difficulties in early detection and longitudinal observation of transgene-induced tumours, which in most cases are randomly located and occur at various time points. Methods such as palpation followed by mechanical measurement of the tumours are of limited value in transgenic models. There is a crucial need for making these previously generated models suitable for modern methods of tumour visualisation and monitoring, e.g. by bioluminescence-based techniques. This approach was successfully used in the current study.ResultsA new mouse strain (MMTV-Luc2 mice) expressing Luc2 luciferase primarily in mammary tissue in females, with low-level background expression in internal organs, was generated and bred to homozygosity. After these mice were intercrossed with MMTV-PyVT mice, all double transgenic females developed mammary tumours by the age of 10 weeks, the localisation and progression of which could be effectively monitored using the luminescence-based in vivo imaging. Luminescence-based readout allowed for early visualisation of the locally overgrown mammary tissue and for longitudinal evaluation of local progression of the tumours. When sampled ex vivo at the age of 10 weeks, all tumours derived from MMTV-Luc2PyVT females displayed robust bioluminescent signal.ConclusionsWe have created a novel transgenic strain for visualisation and longitudinal monitoring of mammary tumour development in transgenic mice as an addition and/or a new and more advanced alternative to manual methods. Generation of this mouse strain is vital for making many of the existing mammary tumour transgenic models applicable for in vivo imaging techniques.
dc.titleGeneration of a new bioluminescent model for visualisation of mammary tumour development in transgenic mice
dc.typeJournal Article
dc.language.rfc3066en
dc.rights.holderAgnieszka M Zagozdzon et al.; licensee BioMed Central Ltd.
dc.description.statusPeer Reviewed
dc.date.updated2012-08-03T15:02:04Z
refterms.dateFOA2018-08-22T19:32:00Z
html.description.abstractAbstractBackgroundNumerous transgenic models have been generated to study breast cancer. However, despite many advantages, traditional transgenic models for breast cancer are also burdened with difficulties in early detection and longitudinal observation of transgene-induced tumours, which in most cases are randomly located and occur at various time points. Methods such as palpation followed by mechanical measurement of the tumours are of limited value in transgenic models. There is a crucial need for making these previously generated models suitable for modern methods of tumour visualisation and monitoring, e.g. by bioluminescence-based techniques. This approach was successfully used in the current study.ResultsA new mouse strain (MMTV-Luc2 mice) expressing Luc2 luciferase primarily in mammary tissue in females, with low-level background expression in internal organs, was generated and bred to homozygosity. After these mice were intercrossed with MMTV-PyVT mice, all double transgenic females developed mammary tumours by the age of 10 weeks, the localisation and progression of which could be effectively monitored using the luminescence-based in vivo imaging. Luminescence-based readout allowed for early visualisation of the locally overgrown mammary tissue and for longitudinal evaluation of local progression of the tumours. When sampled ex vivo at the age of 10 weeks, all tumours derived from MMTV-Luc2PyVT females displayed robust bioluminescent signal.ConclusionsWe have created a novel transgenic strain for visualisation and longitudinal monitoring of mammary tumour development in transgenic mice as an addition and/or a new and more advanced alternative to manual methods. Generation of this mouse strain is vital for making many of the existing mammary tumour transgenic models applicable for in vivo imaging techniques.


Files in this item

Thumbnail
Name:
1471-2407-12-209.xml
Size:
63.17Kb
Format:
XML
Thumbnail
Name:
1471-2407-12-209.pdf
Size:
1.878Mb
Format:
PDF
Thumbnail
Name:
1471-2407-12-209-S1.PDF
Size:
1.579Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record