Modeling ductal carcinoma in situ: a HER2-Notch3 collaboration enables luminal filling.
Authors
Pradeep, C-RKöstler, W J
Lauriola, M
Granit, R Z
Zhang, F
Jacob-Hirsch, J
Rechavi, G
Nair, H B
Hennessy, B T
Gonzalez-Angulo, A M
Tekmal, R R
Ben-Porath, I
Mills, G B
Domany, E
Yarden, Y
Affiliation
Department of Biological Regulation, The Weizmann Institute of Science, Rehovot, Israel.Issue Date
2012-02-16MeSH
AnimalsBreast Neoplasms
Carcinoma, Intraductal, Noninfiltrating
Cell Line
Cell Proliferation
Epidermal Growth Factor
Epithelial Cells
Female
Gene Expression Profiling
Green Fluorescent Proteins
HEK293 Cells
Humans
Immunoblotting
Mammary Glands, Human
Mice
Mice, Transgenic
Microscopy, Confocal
Models, Biological
Oligonucleotide Array Sequence Analysis
RNA Interference
Receptor, erbB-2
Receptors, Notch
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction
Transfection
Metadata
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Modeling ductal carcinoma in situ: a HER2-Notch3 collaboration enables luminal filling. 2012, 31 (7):907-17 OncogeneJournal
OncogeneDOI
10.1038/onc.2011.279PubMed ID
21743488Abstract
A large fraction of ductal carcinoma in situ (DCIS), a non-invasive precursor lesion of invasive breast cancer, overexpresses the HER2/neu oncogene. The ducts of DCIS are abnormally filled with cells that evade apoptosis, but the underlying mechanisms remain incompletely understood. We overexpressed HER2 in mammary epithelial cells and observed growth factor-independent proliferation. When grown in extracellular matrix as three-dimensional spheroids, control cells developed a hollow lumen, but HER2-overexpressing cells populated the lumen by evading apoptosis. We demonstrate that HER2 overexpression in this cellular model of DCIS drives transcriptional upregulation of multiple components of the Notch survival pathway. Importantly, luminal filling required upregulation of a signaling pathway comprising Notch3, its cleaved intracellular domain and the transcriptional regulator HES1, resulting in elevated levels of c-MYC and cyclin D1. In line with HER2-Notch3 collaboration, drugs intercepting either arm reverted the DCIS-like phenotype. In addition, we report upregulation of Notch3 in hyperplastic lesions of HER2 transgenic animals, as well as an association between HER2 levels and expression levels of components of the Notch pathway in tumor specimens of breast cancer patients. Therefore, it is conceivable that the integration of the Notch and HER2 signaling pathways contributes to the pathophysiology of DCIS.Item Type
ArticleLanguage
enISSN
1476-5594ae974a485f413a2113503eed53cd6c53
10.1038/onc.2011.279
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