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dc.contributor.authorSolomon, K
dc.contributor.authorMurray, S
dc.contributor.authorScott, L
dc.contributor.authorMcDermott, S
dc.contributor.authorDrudy, D
dc.contributor.authorMartin, A
dc.contributor.authorO'Donoghue, C
dc.contributor.authorSkally, M
dc.contributor.authorBurns, K
dc.contributor.authorFenelon, L
dc.contributor.authorFitzpatrick, F
dc.contributor.authorKyne, L
dc.contributor.authorFanning, S
dc.date.accessioned2012-06-15T13:26:26Z
dc.date.available2012-06-15T13:26:26Z
dc.date.issued2011-08
dc.identifier.citationAn investigation of the subtype diversity of clinical isolates of Irish Clostridium difficile ribotypes 027 and 078 by repetitive-extragenic palindromic PCR. 2011, 60 (Pt 8):1080-7 J. Med. Microbiol.en_GB
dc.identifier.issn1473-5644
dc.identifier.pmid21459905
dc.identifier.doi10.1099/jmm.0.029983-0
dc.identifier.urihttp://hdl.handle.net/10147/229133
dc.description.abstractA repetitive-extragenic palindromic PCR (rep-PCR) subtyping method (DiversiLab) in conjunction with ribotyping, toxinotyping and antimicrobial-susceptibility testing was used to detect subtypes within Clostridium difficile ribotypes 027 and 078. Clinical isolates of ribotypes 027 (toxinotype III) (n = 30) and 078 (toxinotype V) (n = 23) were provided by health-care facilities across the Republic of Ireland over 2 months in 2006 and 1 month in 2009. Ribotype 027 isolates were significantly more related to each other (9 different subtype profiles) when compared to ribotype 078 isolates (14 different profiles) (P = 0.001; cut-off >90 % similarity). Almost half of ribotype 078 isolates (45.5 %) showed no relationship to each other. The clonality of ribotype 027 isolates suggests effective adaptation to the human niche, whereas the considerable genetic diversity within ribotype 078 isolates suggests that they may have originated from a variety of sources. Subtyping correlated well with antimicrobial susceptibility, in particular clindamycin susceptibility for ribotype 027, but diverse antimicrobial-susceptibility profiles were seen in ribotype 078 isolates, even within a single health-care facility. Between 2006 and 2009, a change in the predominant subtype of ribotype 027 was seen, with the recent clone representing half of all ribotype 027 isolates studied. This strain exhibited 89 % similarity to a rep-PCR profile of the North American NAP-1 strain.
dc.language.isoenen
dc.rightsArchived with thanks to Journal of medical microbiologyen_GB
dc.subject.meshAnti-Bacterial Agents
dc.subject.meshClostridium Infections
dc.subject.meshClostridium difficile
dc.subject.meshDNA, Bacterial
dc.subject.meshDrug Resistance, Bacterial
dc.subject.meshGenetic Variation
dc.subject.meshGenotype
dc.subject.meshHospitals
dc.subject.meshHumans
dc.subject.meshInverted Repeat Sequences
dc.subject.meshIreland
dc.subject.meshPhylogeny
dc.subject.meshPolymerase Chain Reaction
dc.subject.meshRibotyping
dc.subject.meshTime Factors
dc.titleAn investigation of the subtype diversity of clinical isolates of Irish Clostridium difficile ribotypes 027 and 078 by repetitive-extragenic palindromic PCR.en_GB
dc.typeArticleen
dc.contributor.departmentUCD Veterinary Sciences Centre, University College Dublin, Belfield, Dublin 4, Ireland. katie.solomon@ucd.ieen_GB
dc.identifier.journalJournal of medical microbiologyen_GB
dc.description.provinceLeinsteren
html.description.abstractA repetitive-extragenic palindromic PCR (rep-PCR) subtyping method (DiversiLab) in conjunction with ribotyping, toxinotyping and antimicrobial-susceptibility testing was used to detect subtypes within Clostridium difficile ribotypes 027 and 078. Clinical isolates of ribotypes 027 (toxinotype III) (n = 30) and 078 (toxinotype V) (n = 23) were provided by health-care facilities across the Republic of Ireland over 2 months in 2006 and 1 month in 2009. Ribotype 027 isolates were significantly more related to each other (9 different subtype profiles) when compared to ribotype 078 isolates (14 different profiles) (P = 0.001; cut-off >90 % similarity). Almost half of ribotype 078 isolates (45.5 %) showed no relationship to each other. The clonality of ribotype 027 isolates suggests effective adaptation to the human niche, whereas the considerable genetic diversity within ribotype 078 isolates suggests that they may have originated from a variety of sources. Subtyping correlated well with antimicrobial susceptibility, in particular clindamycin susceptibility for ribotype 027, but diverse antimicrobial-susceptibility profiles were seen in ribotype 078 isolates, even within a single health-care facility. Between 2006 and 2009, a change in the predominant subtype of ribotype 027 was seen, with the recent clone representing half of all ribotype 027 isolates studied. This strain exhibited 89 % similarity to a rep-PCR profile of the North American NAP-1 strain.


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