An investigation of the subtype diversity of clinical isolates of Irish Clostridium difficile ribotypes 027 and 078 by repetitive-extragenic palindromic PCR.
Authors
Solomon, KMurray, S
Scott, L
McDermott, S
Drudy, D
Martin, A
O'Donoghue, C
Skally, M
Burns, K
Fenelon, L
Fitzpatrick, F
Kyne, L
Fanning, S
Affiliation
UCD Veterinary Sciences Centre, University College Dublin, Belfield, Dublin 4, Ireland. katie.solomon@ucd.ieIssue Date
2011-08MeSH
Anti-Bacterial AgentsClostridium Infections
Clostridium difficile
DNA, Bacterial
Drug Resistance, Bacterial
Genetic Variation
Genotype
Hospitals
Humans
Inverted Repeat Sequences
Ireland
Phylogeny
Polymerase Chain Reaction
Ribotyping
Time Factors
Metadata
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An investigation of the subtype diversity of clinical isolates of Irish Clostridium difficile ribotypes 027 and 078 by repetitive-extragenic palindromic PCR. 2011, 60 (Pt 8):1080-7 J. Med. Microbiol.Journal
Journal of medical microbiologyDOI
10.1099/jmm.0.029983-0PubMed ID
21459905Abstract
A repetitive-extragenic palindromic PCR (rep-PCR) subtyping method (DiversiLab) in conjunction with ribotyping, toxinotyping and antimicrobial-susceptibility testing was used to detect subtypes within Clostridium difficile ribotypes 027 and 078. Clinical isolates of ribotypes 027 (toxinotype III) (n = 30) and 078 (toxinotype V) (n = 23) were provided by health-care facilities across the Republic of Ireland over 2 months in 2006 and 1 month in 2009. Ribotype 027 isolates were significantly more related to each other (9 different subtype profiles) when compared to ribotype 078 isolates (14 different profiles) (P = 0.001; cut-off >90 % similarity). Almost half of ribotype 078 isolates (45.5 %) showed no relationship to each other. The clonality of ribotype 027 isolates suggests effective adaptation to the human niche, whereas the considerable genetic diversity within ribotype 078 isolates suggests that they may have originated from a variety of sources. Subtyping correlated well with antimicrobial susceptibility, in particular clindamycin susceptibility for ribotype 027, but diverse antimicrobial-susceptibility profiles were seen in ribotype 078 isolates, even within a single health-care facility. Between 2006 and 2009, a change in the predominant subtype of ribotype 027 was seen, with the recent clone representing half of all ribotype 027 isolates studied. This strain exhibited 89 % similarity to a rep-PCR profile of the North American NAP-1 strain.Item Type
ArticleLanguage
enISSN
1473-5644ae974a485f413a2113503eed53cd6c53
10.1099/jmm.0.029983-0
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