Identification of a myometrial molecular profile for dystocic labor.
Authors
Brennan, Donal JMcGee, Sharon F
Rexhepaj, Elton
O'Connor, Darran P
Robson, Michael
O'Herlihy, Colm
Affiliation
National Maternity Hospital, Dublin , Ireland.Issue Date
2011MeSH
AdultAminopeptidases
Case-Control Studies
Cesarean Section
Down-Regulation
Dystocia
Female
Humans
Myometrium
Parity
Polymerase Chain Reaction
Pregnancy
RNA
Uterine Contraction
Metadata
Show full item recordCitation
Identification of a myometrial molecular profile for dystocic labor. 2011, 11:74 BMC Pregnancy ChildbirthJournal
BMC pregnancy and childbirthDOI
10.1186/1471-2393-11-74PubMed ID
21999197Abstract
The most common indication for cesarean section (CS) in nulliparous women is dystocia secondary to ineffective myometrial contractility. The aim of this study was to identify a molecular profile in myometrium associated with dystocic labor.Myometrial biopsies were obtained from the upper incisional margins of nulliparous women undergoing lower segment CS for dystocia (n = 4) and control women undergoing CS in the second stage who had demonstrated efficient uterine action during the first stage of labor (n = 4). All patients were in spontaneous (non-induced) labor and had received intrapartum oxytocin to accelerate labor. RNA was extracted from biopsies and hybridized to Affymetrix HuGene U133A Plus 2 microarrays. Internal validation was performed using quantitative SYBR Green Real-Time PCR.
Seventy genes were differentially expressed between the two groups. 58 genes were down-regulated in the dystocia group. Gene ontology analysis revealed 12 of the 58 down-regulated genes were involved in the immune response. These included (ERAP2, (8.67 fold change (FC)) HLA-DQB1 (7.88 FC) CD28 (2.60 FC), LILRA3 (2.87 FC) and TGFBR3 (2.1 FC)) Hierarchical clustering demonstrated a difference in global gene expression patterns between the samples from dystocic and non-dystocic labours. RT-PCR validation was performed on 4 genes ERAP2, CD28, LILRA3 and TGFBR3
These findings suggest an underlying molecular basis for dystocia in nulliparous women in spontaneous labor. Differentially expressed genes suggest an important role for the immune response in dystocic labor and may provide important indicators for new diagnostic assays and potential intrapartum therapeutic targets.
Language
enISSN
1471-2393Ethical Approval
N/Aae974a485f413a2113503eed53cd6c53
10.1186/1471-2393-11-74
Scopus Count
Collections
Related articles
- Applying a physiologic partograph to Consortium on Safe Labor data to identify opportunities for safely decreasing cesarean births among nulliparous women.
- Authors: Neal JL, Lowe NK, Caughey AB, Bennett KA, Tilden EL, Carlson NS, Phillippi JC, Dietrich MS
- Issue date: 2018 Dec
- Myometrial steroid concentration and oxytocin receptor density in parturient women at term.
- Authors: Rezapour M, Bäckström T, Ulmsten U
- Issue date: 1996 Jun
- Laminin and Collagen IV: Two Polypeptides as Marker of Dystocic Labor.
- Authors: Malvasi A, Cavallotti C, Resta L, Mynbaev OA, Di Tommaso S, Vergara D, Gustapane S, Giacci F, Tinelli A
- Issue date: 2017
- The effect of dystocia and previous cesarean uterine scar on the tensile properties of the lower uterine segment.
- Authors: Buhimschi CS, Buhimschi IA, Yu C, Wang H, Sharer DJ, Diamond MP, Petkova AP, Garfield RE, Saade GR, Weiner CP
- Issue date: 2006 Mar
- Dystocic Labor and Adrenergic and Noradrenergic Neurotransmitters: A Morphological Experimental Study.
- Authors: Malvasi A, Vimercati A, Ricci I, Picardi N, Cicinelli E, Kosmas I, Baldini GM, Tinelli A
- Issue date: 2022 Sep 27