Effects of Long-term exposure of Gelatinated and Non-gelatinated Cadmium Telluride Quantum Dots on Differentiated PC12 cells
dc.contributor.author | Prasad, Babu R | |
dc.contributor.author | Mullins, Gillian | |
dc.contributor.author | Nikolskaya, Natalia | |
dc.contributor.author | Connolly, David | |
dc.contributor.author | Smith, Terry J. | |
dc.contributor.author | Gerard, Valerie A. | |
dc.contributor.author | Byrne, Stephen J. | |
dc.contributor.author | Davies, Gemma-Louise | |
dc.contributor.author | Gun'ko, Yurii K. | |
dc.contributor.author | Rochev, Yury | |
dc.date.accessioned | 2012-03-09T12:50:23Z | |
dc.date.available | 2012-03-09T12:50:23Z | |
dc.date.issued | 2012-01-20 | |
dc.identifier | http://dx.doi.org/10.1186/1477-3155-10-4 | |
dc.identifier.citation | Journal of Nanobiotechnology. 2012 Jan 20;10(1):4 | |
dc.identifier.uri | http://hdl.handle.net/10147/215154 | |
dc.description.abstract | Abstract Background The inherent toxicity of unmodified Quantum Dots (QDs) is a major hindrance to their use in biological applications. To make them more potent as neuroprosthetic and neurotherapeutic agents, thioglycolic acid (TGA) capped CdTe QDs, were coated with a gelatine layer and investigated in this study with differentiated pheochromocytoma 12 (PC12) cells. The QD - cell interactions were investigated after incubation periods of up to 17 days by MTT and APOTOX-Glo Triplex assays along with using confocal microscopy. Results Long term exposure (up to 17 days) to gelatinated TGA-capped CdTe QDs of PC12 cells in the course of differentiation and after neurites were grown resulted in dramatically reduced cytotoxicity compared to non-gelatinated TGA-capped CdTe QDs. Conclusion The toxicity mechanism of QDs was identified as caspase-mediated apoptosis as a result of cadmium leaking from the core of QDs. It was therefore concluded that the gelatine capping on the surface of QDs acts as a barrier towards the leaking of toxic ions from the core QDs in the long term (up to 17 days). | |
dc.title | Effects of Long-term exposure of Gelatinated and Non-gelatinated Cadmium Telluride Quantum Dots on Differentiated PC12 cells | |
dc.type | Journal Article | |
dc.language.rfc3066 | en | |
dc.rights.holder | Prasad et al.; licensee BioMed Central Ltd. | |
dc.description.status | Peer Reviewed | |
dc.date.updated | 2012-03-04T16:01:58Z | |
refterms.dateFOA | 2018-08-22T16:22:38Z | |
html.description.abstract | Abstract Background The inherent toxicity of unmodified Quantum Dots (QDs) is a major hindrance to their use in biological applications. To make them more potent as neuroprosthetic and neurotherapeutic agents, thioglycolic acid (TGA) capped CdTe QDs, were coated with a gelatine layer and investigated in this study with differentiated pheochromocytoma 12 (PC12) cells. The QD - cell interactions were investigated after incubation periods of up to 17 days by MTT and APOTOX-Glo Triplex assays along with using confocal microscopy. Results Long term exposure (up to 17 days) to gelatinated TGA-capped CdTe QDs of PC12 cells in the course of differentiation and after neurites were grown resulted in dramatically reduced cytotoxicity compared to non-gelatinated TGA-capped CdTe QDs. Conclusion The toxicity mechanism of QDs was identified as caspase-mediated apoptosis as a result of cadmium leaking from the core of QDs. It was therefore concluded that the gelatine capping on the surface of QDs acts as a barrier towards the leaking of toxic ions from the core QDs in the long term (up to 17 days). |