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dc.contributor.authorPrasad, Babu R
dc.contributor.authorMullins, Gillian
dc.contributor.authorNikolskaya, Natalia
dc.contributor.authorConnolly, David
dc.contributor.authorSmith, Terry J.
dc.contributor.authorGerard, Valerie A.
dc.contributor.authorByrne, Stephen J.
dc.contributor.authorDavies, Gemma-Louise
dc.contributor.authorGun'ko, Yurii K.
dc.contributor.authorRochev, Yury
dc.date.accessioned2012-03-09T12:50:23Z
dc.date.available2012-03-09T12:50:23Z
dc.date.issued2012-01-20
dc.identifierhttp://dx.doi.org/10.1186/1477-3155-10-4
dc.identifier.citationJournal of Nanobiotechnology. 2012 Jan 20;10(1):4
dc.identifier.urihttp://hdl.handle.net/10147/215154
dc.description.abstractAbstract Background The inherent toxicity of unmodified Quantum Dots (QDs) is a major hindrance to their use in biological applications. To make them more potent as neuroprosthetic and neurotherapeutic agents, thioglycolic acid (TGA) capped CdTe QDs, were coated with a gelatine layer and investigated in this study with differentiated pheochromocytoma 12 (PC12) cells. The QD - cell interactions were investigated after incubation periods of up to 17 days by MTT and APOTOX-Glo Triplex assays along with using confocal microscopy. Results Long term exposure (up to 17 days) to gelatinated TGA-capped CdTe QDs of PC12 cells in the course of differentiation and after neurites were grown resulted in dramatically reduced cytotoxicity compared to non-gelatinated TGA-capped CdTe QDs. Conclusion The toxicity mechanism of QDs was identified as caspase-mediated apoptosis as a result of cadmium leaking from the core of QDs. It was therefore concluded that the gelatine capping on the surface of QDs acts as a barrier towards the leaking of toxic ions from the core QDs in the long term (up to 17 days).
dc.titleEffects of Long-term exposure of Gelatinated and Non-gelatinated Cadmium Telluride Quantum Dots on Differentiated PC12 cells
dc.typeJournal Article
dc.language.rfc3066en
dc.rights.holderPrasad et al.; licensee BioMed Central Ltd.
dc.description.statusPeer Reviewed
dc.date.updated2012-03-04T16:01:58Z
refterms.dateFOA2018-08-22T16:22:38Z
html.description.abstractAbstract Background The inherent toxicity of unmodified Quantum Dots (QDs) is a major hindrance to their use in biological applications. To make them more potent as neuroprosthetic and neurotherapeutic agents, thioglycolic acid (TGA) capped CdTe QDs, were coated with a gelatine layer and investigated in this study with differentiated pheochromocytoma 12 (PC12) cells. The QD - cell interactions were investigated after incubation periods of up to 17 days by MTT and APOTOX-Glo Triplex assays along with using confocal microscopy. Results Long term exposure (up to 17 days) to gelatinated TGA-capped CdTe QDs of PC12 cells in the course of differentiation and after neurites were grown resulted in dramatically reduced cytotoxicity compared to non-gelatinated TGA-capped CdTe QDs. Conclusion The toxicity mechanism of QDs was identified as caspase-mediated apoptosis as a result of cadmium leaking from the core of QDs. It was therefore concluded that the gelatine capping on the surface of QDs acts as a barrier towards the leaking of toxic ions from the core QDs in the long term (up to 17 days).


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