Show simple item record

dc.contributor.authorMagdalon, Juliana
dc.contributor.authorHatanaka, Elaine
dc.contributor.authorRomanatto, Talita
dc.contributor.authorRodrigues, Hosana G
dc.contributor.authorKuwabara, Wilson MT
dc.contributor.authorScaife, Caitriona
dc.contributor.authorNewsholme, Philip
dc.contributor.authorCuri, Rui
dc.date.accessioned2012-02-21T12:29:39Z
dc.date.available2012-02-21T12:29:39Z
dc.date.issued2011-11-24
dc.identifierhttp://dx.doi.org/10.1186/1476-511X-10-218
dc.identifier.citationLipids in Health and Disease. 2011 Nov 24;10(1):218
dc.identifier.urihttp://hdl.handle.net/10147/211869
dc.description.abstractAbstract Previous studies have demonstrated that long chain fatty acids influence fibroblast function at sub-lethal concentrations. This study is the first to assess the effects of oleic, linoleic or palmitic acids on protein expression of fibroblasts, as determined by standard proteomic techniques. The fatty acids were not cytotoxic at the concentration used in this work as assessed by membrane integrity, DNA fragmentation and the MTT assay but significantly increased cell proliferation. Subsequently, a proteomic analysis was performed using two dimensional difference gel electrophoresis (2D-DIGE) and MS based identification. Cells treated with 50 μM oleic, linoleic or palmitic acid for 24 h were associated with 24, 22, 16 spots differentially expressed, respectively. Among the identified proteins, α-enolase and far upstream element binding protein 1 (FBP-1) are of importance due to their function in fibroblast-associated diseases. However, modulation of α-enolase and FBP-1 expression by fatty acids was not validated by the Western blot technique.
dc.titleA proteomic analysis of the functional effects of fatty acids in NIH 3T3 fibroblasts
dc.typeJournal Article
dc.language.rfc3066en
dc.rights.holderMagdalon et al.; licensee BioMed Central Ltd.
dc.description.statusPeer Reviewed
dc.date.updated2012-02-17T20:10:04Z
refterms.dateFOA2018-08-22T15:52:56Z
html.description.abstractAbstract Previous studies have demonstrated that long chain fatty acids influence fibroblast function at sub-lethal concentrations. This study is the first to assess the effects of oleic, linoleic or palmitic acids on protein expression of fibroblasts, as determined by standard proteomic techniques. The fatty acids were not cytotoxic at the concentration used in this work as assessed by membrane integrity, DNA fragmentation and the MTT assay but significantly increased cell proliferation. Subsequently, a proteomic analysis was performed using two dimensional difference gel electrophoresis (2D-DIGE) and MS based identification. Cells treated with 50 μM oleic, linoleic or palmitic acid for 24 h were associated with 24, 22, 16 spots differentially expressed, respectively. Among the identified proteins, α-enolase and far upstream element binding protein 1 (FBP-1) are of importance due to their function in fibroblast-associated diseases. However, modulation of α-enolase and FBP-1 expression by fatty acids was not validated by the Western blot technique.


Files in this item

Thumbnail
Name:
1476-511X-10-218.xml
Size:
60.47Kb
Format:
XML
Thumbnail
Name:
1476-511X-10-218.pdf
Size:
631.4Kb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record