Interpretive criteria for mupirocin susceptibility testing of Staphylococcus spp. using CLSI guidelines.
AffiliationDepartment of Medical Microbiology, Cork University Hospital, Wilton, Cork,, Ireland.
*Drug Resistance, Bacterial
Microbial Sensitivity Tests/*methods
Multicenter Studies as Topic
Staphylococcal Infections/*drug therapy
Staphylococcus aureus/*drug effects/growth & development
MetadataShow full item record
CitationBr J Biomed Sci. 2007;64(1):1-5.
JournalBritish journal of biomedical science
AbstractMupirocin is an antimicrobial agent commonly used to treat staphylococcal infection or to eliminate persistent carriage. To date, interpretive criteria have not been established to define susceptibility or resistance when performing mupirocin susceptibility testing. In this evaluation, using CLSI guidelines, a total of 502 staphylococci comprising 219 methicillin-sensitive Staphylococcus aureus, 222 methicillin-resistant S. aureus and 61 coagulase-negative staphylococci are tested by broth microdilution, disc diffusion and E-test. Disc diffusion using 5 microg mupirocin discs was found to be a reliable method to distinguish susceptible and resistant strains. Minimum inhibitory concentration (MIC) determination was required to differentiate low-level and high-level resistance to mupirocin. E-test was found to be an accurate alternative to broth microdilution for the routine determination of MIC values of staphylococci to mupirocin. Broth microdilution and disc-diffusion results were plotted on a scattergram, and error rates were calculated. No errors were found using susceptibility criteria of < 4 microg/mL (MIC) and > 19 mm (zone diameter).
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