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    The effect of lactase and formula reconstitution on milk osmolality.

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    Authors
    Malone, A J
    Kearney, P J
    Duggan, P F
    Affiliation
    Department of Paediatrics and Child Health, Cork University Hospital, Ireland.
    Issue Date
    2012-02-03T15:13:00Z
    MeSH
    Animals
    Humans
    Infant
    Infant Food/*analysis
    Lactase
    Lactose/metabolism
    Milk/*chemistry/drug effects
    Osmolar Concentration
    Time Factors
    beta-Galactosidase/*physiology
    
    Metadata
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    Citation
    Int J Food Sci Nutr. 1999 Sep;50(5):311-7.
    Journal
    International journal of food sciences and nutrition
    URI
    http://hdl.handle.net/10147/209128
    PubMed ID
    10719561
    Abstract
    These experiments investigated the reaction rate of lactase on milk lactose by measuring milk osmolality; and explored the effect of formula reconstitution on milk osmolality. The investigations measured milk osmolality with the Fiske Os, freezing-point osmometer. Lactase (Lactaid) incubated with pure lactose solutions established the validity of the method. Lactase was incubated for 24 hours with four reconstituted milk formulas (Milumil, and Cow and Gate Nutrilon Plus, Farley's First Milk, SMA Gold). Milk osmolality increased most rapidly in the first 4 hours after the addition of lactase. The lactase enzyme completed over 90% of the reaction within 12 hours. The milk osmolalities ranged from 487 to 591 mosm/kg after 24 hours with 2-4 drops of lactase in 240 ml of formula. A clinical guideline osmolality of 400 mosm/kg was reached in 240 ml of formula at 1 to 12 hours depending on the dose of lactase. High milk osmolalities due to prolonged enzyme incubation, or high lactase doses could be reduced to around 400 mosm/kg by dilution of 240 ml of formula with an extra 60 ml of water. The initial osmolality of formula after reconstitution by paediatric nurses varied widely and usually exceeded the manufacturer's quoted osmolality. This initial osmolality was a further influence on the final osmolality reached after the addition of lactase. It is concluded that the recommended incubation time for Lactaid of 24 hours is unnecessary as lactase exerts the majority of its effect in less than 12 hours. Adjustment of Lactaid dose and incubation times will maintain milk formula osmolality within standard guidelines. Dilution with extra water will correct inadvertent high enzyme doses and prolonged incubation times. The normal method of reconstituting milk formulas from powder may be unreliable as the manufacturer's quoted osmolality was not reproduced when milk formulas were reconstituted by paediatric nurses.
    Language
    eng
    ISSN
    0963-7486 (Print)
    0963-7486 (Linking)
    Collections
    Cork University Hospital

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