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    Neutrophil-induced transmigration of tumour cells treated with tumour-conditioned medium is facilitated by granulocyte-macrophage colony-stimulating factor.

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    Authors
    Wu, Q D
    Wang, J H
    Bouchier-Hayes, D
    Redmond, H P
    Affiliation
    Department of Surgery, Cork University Hospital, University College Cork,, Ireland.
    Issue Date
    2012-02-03T15:12:41Z
    MeSH
    Breast Neoplasms/*pathology/physiopathology
    Cell Adhesion Molecules
    Cell Movement
    Culture Media
    Cytokines/physiology
    Endothelium, Vascular
    Female
    Granulocyte-Macrophage Colony-Stimulating Factor/*physiology
    Humans
    Interleukin-3/physiology
    Interleukin-8/physiology
    Neutrophils/*physiology
    Tumor Cells, Cultured/*physiology
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    Citation
    Eur J Surg. 2000 May;166(5):361-6.
    Journal
    The European journal of surgery = Acta chirurgica
    URI
    http://hdl.handle.net/10147/209116
    DOI
    10.1080/110241500750008899
    PubMed ID
    10881945
    Abstract
    OBJECTIVE: To investigate the effect of different cytokines that are present in tumour-conditioned medium on human neutrophil (PMN)-induced tumour cell transmigration. DESIGN: Laboratory study. SETTING: University hospital, Ireland. MATERIAL: Isolated human PMN and cultured human breast tumour cell line, MDA-MB-231. Interventions: Human PMN treated with either tumour-conditioned medium or different media neutralised with monoclonal antibodies (MoAb), and MDA-MB-231 cells were plated on macrovascular and microvascular endothelial monolayers in collagen-coated transwells to assess migration of tumour cells. MAIN OUTCOME MEASURES: Cytokines present in tumour-conditioned medium, PMN cytocidal function and receptor expression, and tumour cell transmigration. RESULTS: tumour-conditioned medium contained high concentrations of granulocyte-macrophage colony-stimulating factor (GM-CSF), vascular endothelial growth factor (VEGF), and interleukin 8 (IL-8), but not granulocyte colony-stimulating factor (G-CSF) and interleukin 3 (IL-3). Anti-GM-CSF MoAb significantly reduced PMN-induced transmigration of tumour cells treated with tumour-conditioned medium (p < 0.05), whereas anti-VEGF and anti-IL-8 MoAbs did not affect their migration. In addition, anti-GM-CSF MoAb, but not anti-VEGF or anti-IL-8 MoAb, reduced PMN CD11b and CD18 overexpression induced by tumour-conditioned medium (p < 0.05). CONCLUSION: These results indicate that the GM-CSF that is present in tumour-conditioned medium may be involved, at least in part, in alterations in PMN function mediated by the medium and subsequently PMN-induced transmigration of tumour cells.
    Language
    eng
    ISSN
    1102-4151 (Print)
    1102-4151 (Linking)
    ae974a485f413a2113503eed53cd6c53
    10.1080/110241500750008899
    Scopus Count
    Collections
    Cork University Hospital

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