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    Strategy for the maximization of clinically relevant information from hepatitis C virus, RT-PCR quantification.

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    Authors
    Levis, J
    Kenny-Walsh, E
    O'Sullivan, K
    Horgan, M
    Whelton, M
    Shanahan, F
    Fanning, L
    Affiliation
    Hepatitis C Unit, Department of Medicine, Clinical Sciences Building, Cork, University Hospital, University College, Cork, Ireland.
    Issue Date
    2012-02-03T15:12:13Z
    MeSH
    DNA, Viral/analysis
    Enzyme-Linked Immunosorbent Assay
    Female
    Genotype
    Hepacivirus/*genetics
    Hepatitis C/*virology
    Humans
    Male
    Prospective Studies
    RNA, Viral/blood
    Reagent Kits, Diagnostic
    Retrospective Studies
    Reverse Transcriptase Polymerase Chain Reaction
    Viral Load
    Viremia/virology
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    Citation
    J Clin Virol. 2001 Feb;20(3):163-71.
    Journal
    Journal of clinical virology : the official publication of the Pan American, Society for Clinical Virology
    URI
    http://hdl.handle.net/10147/209098
    PubMed ID
    11166666
    Abstract
    BACKGROUND: The increasing clinical application of viral load assays for monitoring viral infections has been an incentive for the development of standardized tests for the hepatitis C virus. OBJECTIVE: To develop a simple model for the prediction of baseline viral load in individuals infected with the hepatitis C virus. METHODOLOGY: Viral load quantification of each patient's first sample was assessed by RT-PCR-ELISA using the Roche MONITOR assay in triplicate. Genotype of the infecting virus was identified by reverse line probe hybridization, using amplicons resulting from the qualitative HCV Roche AMPLICOR assay. RESULTS: Retrospective evaluation of first quantitative values suggested that 82.4% (n=168/204) of individuals had a viral load between 4.3 and 6.7 log(10) viral copies per ml. A few patients (3.4%; n=7/204) have a serum viremia less than the lower limit of the linear range of the RT-PCR assay. Subsequent, prospective evaluation of hepatitis C viral load of all new patients using a model based on the dynamic range of viral load in the retrospective group correctly predicted the dynamic range in 75.9% (n=33/54). CONCLUSION: The dynamic range of hepatitis C viremia extends beyond the linear range of the Roche MONITOR assay. Accurate determination of serum viremia is substantially improved by dilution of specimens prior to quantification.
    Language
    eng
    ISSN
    1386-6532 (Print)
    1386-6532 (Linking)
    Collections
    Cork University Hospital

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