Adenosine inhibits neutrophil vascular endothelial growth factor release and transendothelial migration via A2B receptor activation.
AffiliationDepartment of Academic Surgery, Cork University Hospital, Cork, Republic of, Ireland.
MeSHAdenosine/*analogs & derivatives/*pharmacology
Cell Movement/drug effects
Cells, Cultured/drug effects
Chemotaxis, Leukocyte/*drug effects
Dose-Response Relationship, Drug
Endothelial Growth Factors/*secretion
Intercellular Adhesion Molecule-1/biosynthesis
Purinergic P1 Receptor Agonists
Purinergic P1 Receptor Antagonists
Receptor, Adenosine A2B
Receptors, Purinergic P1/*drug effects/physiology
Theobromine/analogs & derivatives/pharmacology
Tumor Necrosis Factor-alpha/genetics/pharmacology
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors
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CitationShock. 2001 Apr;15(4):297-301.
JournalShock (Augusta, Ga.)
AbstractThe effects of adenosine on neutrophil (polymorphonuclear neutrophils; PMN)-directed changes in vascular permeability are poorly characterized. This study investigated whether adenosine modulates activated PMN vascular endothelial growth factor (vascular permeability factor; VEGF) release and transendothelial migration. PMN activated with tumour necrosis factor-alpha (TNF-alpha, 10 ng/mL) were incubated with adenosine and its receptor-specific analogues. Culture supernatants were assayed for VEGF. PMN transendothelial migration across human umbilical vein endothelial cell (HUVEC) monolayers was assessed in vitro. Adhesion molecule receptor expression was assessed flow cytometrically. Adenosine and some of its receptor-specific analogues dose-dependently inhibited activated PMN VEGF release. The rank order of potency was consistent with the affinity profile of human A2B receptors. The inhibitory effect of adenosine was reversed by 3,7-dimethyl-1-propargylxanthine, an A2 receptor antagonist. Adenosine (100 microM) or the A2B receptor agonist 5'-N-ethylcarboxamidoadenosine (NECA, 100 microM) significantly reduced PMN transendothelial migration. However, expression of activated PMN beta2 integrins and HUVEC ICAM-1 were not significantly altered by adenosine or NECA. Adenosine attenuates human PMN VEGF release and transendothelial migration via the A2B receptor. This provides a novel target for the modulation of PMN-directed vascular hyperpermeability in conditions such as the capillary leak syndrome.