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dc.contributor.authorAndrews, E J
dc.contributor.authorWang, J H
dc.contributor.authorWinter, D C
dc.contributor.authorLaug, W E
dc.contributor.authorRedmond, H P
dc.date.accessioned2012-02-03T15:11:47Z
dc.date.available2012-02-03T15:11:47Z
dc.date.issued2012-02-03T15:11:47Z
dc.identifier.citationJ Surg Res. 2001 May 1;97(1):14-9.en_GB
dc.identifier.issn0022-4804 (Print)en_GB
dc.identifier.issn0022-4804 (Linking)en_GB
dc.identifier.pmid11319874en_GB
dc.identifier.doi10.1006/jsre.2001.6090en_GB
dc.identifier.urihttp://hdl.handle.net/10147/209082
dc.description.abstractBACKGROUND: Recent studies have demonstrated that metastatic disease develops from tumor cells that adhere to endothelial cells and proliferate intravascularly. The beta-1 integrin family and its ligand laminin have been shown to be important in tumor-to-endothelial cell adhesion. Lipopolysaccharide (LPS) has been implicated in the increased metastatic tumor growth that is seen postoperatively. We postulated that LPS increases tumor cell expression of beta-1 integrins and that this leads to increased adhesion. METHODS: The human metastatic colon cancer cell line LS174T was labeled with an enhanced green fluorescent protein (eGFP) using retroviral transfection. Cell cultures were treated with LPS for 1, 2, and 4 h (n = 6 each) and were subsequently cocultured for 30 or 120 min with confluent human umbilical vein endothelial cells (HUVECs), to allow adherence. Adherent tumor cells were counted using fluorescence microscopy. These experiments were carried out in the presence or absence of a functional blocking beta-1 integrin monoclonal antibody (4B4). Expression of beta-1 integrin and laminin on tumor and HUVECs was assessed using flow cytometric analysis. Tumor cell NF-kappaB activation after incubation with LPS was measured. RESULTS: Tumor cell and HUVEC beta-1 integrin expression and HUVEC expression of laminin were significantly (P < 0.05) enhanced after incubation with LPS. Tumor cell adhesion to HUVECs was significantly increased. Addition of the beta-1 integrin blocking antibody reduced tumor cell adhesion to control levels. LPS increased tumor cell NF-kappaB activation. CONCLUSIONS: Exposure to LPS increases tumor cell adhesion to the endothelium through a beta-1 integrin-mediated pathway that is NF-kappaB dependent. This may provide a target for immunotherapy directed at reducing postoperative metastatic tumor growth.
dc.language.isoengen_GB
dc.subject.meshAntigens, CD29/*biosynthesisen_GB
dc.subject.meshCell Adhesion/*drug effectsen_GB
dc.subject.meshCells, Cultureden_GB
dc.subject.meshEndothelium, Vascular/drug effects/*metabolismen_GB
dc.subject.meshHumansen_GB
dc.subject.meshKineticsen_GB
dc.subject.meshLaminin/biosynthesisen_GB
dc.subject.meshLipopolysaccharides/*pharmacologyen_GB
dc.subject.meshNF-kappa B/antagonists & inhibitors/metabolismen_GB
dc.subject.meshNeoplasm Metastasisen_GB
dc.subject.meshNeoplasms/*metabolism/pathologyen_GB
dc.subject.meshPeptides/pharmacologyen_GB
dc.subject.meshTumor Cells, Cultureden_GB
dc.subject.meshUp-Regulationen_GB
dc.titleTumor cell adhesion to endothelial cells is increased by endotoxin via an upregulation of beta-1 integrin expression.en_GB
dc.contributor.departmentDepartment of Surgery, Cork University Hospital, Cork, Ireland., emmetandrews@eircom.neten_GB
dc.identifier.journalThe Journal of surgical researchen_GB
dc.description.provinceMunster
html.description.abstractBACKGROUND: Recent studies have demonstrated that metastatic disease develops from tumor cells that adhere to endothelial cells and proliferate intravascularly. The beta-1 integrin family and its ligand laminin have been shown to be important in tumor-to-endothelial cell adhesion. Lipopolysaccharide (LPS) has been implicated in the increased metastatic tumor growth that is seen postoperatively. We postulated that LPS increases tumor cell expression of beta-1 integrins and that this leads to increased adhesion. METHODS: The human metastatic colon cancer cell line LS174T was labeled with an enhanced green fluorescent protein (eGFP) using retroviral transfection. Cell cultures were treated with LPS for 1, 2, and 4 h (n = 6 each) and were subsequently cocultured for 30 or 120 min with confluent human umbilical vein endothelial cells (HUVECs), to allow adherence. Adherent tumor cells were counted using fluorescence microscopy. These experiments were carried out in the presence or absence of a functional blocking beta-1 integrin monoclonal antibody (4B4). Expression of beta-1 integrin and laminin on tumor and HUVECs was assessed using flow cytometric analysis. Tumor cell NF-kappaB activation after incubation with LPS was measured. RESULTS: Tumor cell and HUVEC beta-1 integrin expression and HUVEC expression of laminin were significantly (P < 0.05) enhanced after incubation with LPS. Tumor cell adhesion to HUVECs was significantly increased. Addition of the beta-1 integrin blocking antibody reduced tumor cell adhesion to control levels. LPS increased tumor cell NF-kappaB activation. CONCLUSIONS: Exposure to LPS increases tumor cell adhesion to the endothelium through a beta-1 integrin-mediated pathway that is NF-kappaB dependent. This may provide a target for immunotherapy directed at reducing postoperative metastatic tumor growth.


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