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    The effect of the anaesthetic agent isoflurane on the rate of neutrophil apoptosis in vitro.

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    Authors
    Tyther, R
    Fanning, N
    Halligan, M
    Wang, J
    Redmond, H P
    Shorten, G
    Affiliation
    Department of Anaesthesia and Intensive Care Medicine, Cork University Hospital, , University College Cork, Ireland.
    Issue Date
    2012-02-03T15:11:33Z
    MeSH
    Adult
    Anesthetics, Inhalation/*pharmacology
    Apoptosis/*drug effects
    Cells, Cultured
    Flow Cytometry
    Humans
    Isoflurane/*pharmacology
    Neutrophils/*drug effects
    Time Factors
    
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    Citation
    Ir J Med Sci. 2001 Jan-Mar;170(1):41-4.
    Journal
    Irish journal of medical science
    URI
    http://hdl.handle.net/10147/209073
    PubMed ID
    11440412
    Abstract
    BACKGROUND: Volatile anaesthetic agents influence neutrophil function, and potentially, the inflammatory response to surgery. AIM: The objective of this study was to determine the effect of isoflurane (1-4%) on human polymorphonuclear neutrophil apoptosis in vitro. METHODS: Venous blood from 12 healthy volunteers was exposed to 0, 1, and 4% isoflurane delivered via a 14G Wallace flexihub internal jugular cannula, at a fresh gas flow of 0.51/min for 5 minutes. Isolated neutrophils were assessed for apoptosis at 1, 12, and 24 hours in culture using dual staining with annexin V-FITC and propidium iodide (Annexin-V FITC assay). Data were analysed using paired, one-tailed Student's t-tests. p<0.05 was considered significant. RESULTS: At 1 hour apoptosis was inhibited in the 1% (5.1 [6.8]%; p=0.017) and 4% (4.8 [4.5]%; p=0.008) isoflurane groups compared to control (11.3 [6.9]%). At 12 and 24 hours, a dose-dependent inhibition of apoptosis was demonstrated, i.e. 4% > 1% > 0%. CONCLUSION: Human neutrophil apoptosis is inhibited in a concentration-dependent manner in vitro by isoflurane in clinical concentrations.
    Language
    eng
    ISSN
    0021-1265 (Print)
    0021-1265 (Linking)
    Collections
    Cork University Hospital

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