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    Hypertonic saline impedes tumor cell-endothelial cell interaction by reducing adhesion molecule and laminin expression.

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    Authors
    Shields, Conor J
    Winter, Desmond C
    Wang, Jiang Huai
    Andrews, Emmet
    Laug, Walter E
    Redmond, H Paul
    Affiliation
    Department of Academic Surgery, Cork University Hospital and National University , of Ireland, Wilton.
    Issue Date
    2012-02-03T15:08:21Z
    MeSH
    Cell Adhesion/*drug effects
    Cell Adhesion Molecules/biosynthesis
    Cell Communication/*drug effects
    Cell Line, Tumor
    Colonic Neoplasms/*physiopathology
    Endothelial Cells/*drug effects
    Humans
    Laminin/biosynthesis
    Saline Solution, Hypertonic/*pharmacology
    
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    Citation
    Surgery. 2004 Jul;136(1):76-83.
    Journal
    Surgery
    URI
    http://hdl.handle.net/10147/208953
    DOI
    10.1016/j.surg.2003.11.011
    PubMed ID
    15232542
    Abstract
    BACKGROUND: Hypertonic saline infusion dampens inflammatory responses and suppresses neutrophil-endothelial interaction by reducing adhesion molecule expression. This study tested the hypothesis that hypertonic saline attenuates tumor cell adhesion to the endothelium through a similar mechanism. METHODS: Human colon cancer cells (LS174T) were transfected with green fluorescent protein and exposed to lipopolysaccharide, tumor necrosis factor-alpha, and interleukin-6 under hypertonic and isotonic conditions for 1 and 4 hours. Confluent human umbilical vein endothelial cells were similarly exposed. Cellular apoptosis and expression of adhesion molecules and laminin were measured by flow cytometry. Tumor cell adhesion to endothelium and laminin was assessed with fluorescence microscopy. Data are represented as mean +/- standard error of mean, and an ANOVA test was performed to gauge statistical significance, with P <.05 considered significant. RESULTS: Hypertonic exposure significantly reduced tumor cell adhesion despite the presence of the perioperative cell stressors (42 +/- 2.9 vs 172.5 +/- 12.4, P <.05), attenuated tumor cell beta-1 integrin (14.43 vs 23.84, P <.05), and endothelial cell laminin expression (22.78 +/- 2.2 vs 33.74 +/- 2.4, P <.05), but did not significantly alter cell viability. CONCLUSION: Hypertonic saline significantly attenuates tumor cell adhesion to endothelium by inhibiting adhesion molecule and laminin expression. This may halt the metastatic behavior of tumor cells shed at surgery.
    Language
    eng
    ISSN
    0039-6060 (Print)
    0039-6060 (Linking)
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.surg.2003.11.011
    Scopus Count
    Collections
    Cork University Hospital

    entitlement

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