• Login
    View Item 
    •   Home
    • Hospital Research
    • Munster
    • Cork University Hospital
    • View Item
    •   Home
    • Hospital Research
    • Munster
    • Cork University Hospital
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Map of Submissions

    Home Page
    UlsterN
    4995
    UlsterS
    4995
    Connacht
    1672
    Munster
    54
    Leinster
    456

    Browse

    All of Lenus, The Irish Health RepositoryCommunitiesTitleAuthorsDate publishedSubjectsThis CollectionTitleAuthorsDate publishedSubjects

    My Account

    LoginRegister

    About

    About LenusDirectory of Open Access JournalsOpen Access Publishing GuideNational Health Library & Knowledge ServiceGuide to Publishers' PoliciesFAQsTerms and ConditionsVision StatementORCID Unique identifiers for ResearchersHSE position statement on Open AccessNational Open Research Forum (NORF)Zenodo (European Open Research repository)

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Mechanisms driving local breast cancer recurrence in a model of breast-conserving surgery.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Smith, Myles J
    Culhane, Aedin C
    Killeen, Shane
    Kelly, Maura A
    Wang, Jiang H
    Cotter, Thomas G
    Redmond, Henry P
    Affiliation
    Department of Academic Surgery, Cork University Hospital, Cork, Ireland., mylessmith@hotmail.com
    Issue Date
    2012-02-03T15:06:43Z
    MeSH
    Animals
    Blotting, Western
    Bone Marrow/metabolism
    Breast Neoplasms/metabolism/*pathology/surgery
    Cancer Vaccines/therapeutic use
    Dendritic Cells/immunology/metabolism
    *Disease Models, Animal
    Female
    Gene Expression Profiling
    Heat-Shock Proteins/therapeutic use
    Humans
    Mice
    Mice, Inbred BALB C
    Neoplasm Recurrence, Local/*diagnosis/metabolism
    Oligonucleotide Array Sequence Analysis
    Phosphatidylinositol 3-Kinases/metabolism
    Phosphorylation
    Proto-Oncogene Proteins c-akt/metabolism
    RNA, Messenger/genetics/metabolism
    Reverse Transcriptase Polymerase Chain Reaction
    Signal Transduction
    Survival Rate
    Tumor Cells, Cultured
    Tumor Markers, Biological/genetics/metabolism
    Show allShow less
    
    Metadata
    Show full item record
    Citation
    Ann Surg Oncol. 2008 Oct;15(10):2954-64. Epub 2008 Jul 12.
    Journal
    Annals of surgical oncology
    URI
    http://hdl.handle.net/10147/208895
    DOI
    10.1245/s10434-008-0037-5
    PubMed ID
    18622646
    Abstract
    OBJECTIVE: We aimed to identify mechanisms driving local recurrence in a model of breast-conserving surgery (BCS) for breast cancer. BACKGROUND: Breast cancer recurrence after BCS remains a clinically significant, but poorly understood problem. We have previously reported that recurrent colorectal tumours demonstrate altered growth dynamics, increased metastatic burden and resistance to apoptosis, mediated by upregulation of phosphoinositide-3-kinase/Akt (PI3K/Akt). We investigated whether similar characteristics were evident in a model of locally recurrent breast cancer. METHODS: Tumours were generated by orthotopic inoculation of 4T1 cells in two groups of female Balb/c mice and cytoreductive surgery performed when mean tumour size was above 150 mm(3). Local recurrence was observed and gene expression was examined using Affymetrix GeneChips in primary and recurrent tumours. Differential expression was confirmed with quantitative real-time polymerase chain reaction (qRT-PCR). Phosphorylation of Akt was assessed using Western immunoblotting. An ex vivo heat shock protein (HSP)-loaded dendritic cell vaccine was administered in the perioperative period. RESULTS: We observed a significant difference in the recurrent 4T1 tumour volume and growth rate (p < 0.05). Gene expression studies suggested roles for the PI3K/Akt system and local immunosuppression driving the altered growth kinetics. We demonstrated that perioperative vaccination with an ex vivo HSP-loaded dendritic cell vaccine abrogated recurrent tumour growth in vivo (p = 0.003 at day 15). CONCLUSION: Investigating therapies which target tumour survival pathways such as PI3K/Akt and boost immune surveillance in the perioperative period may be useful adjuncts to contemporary breast cancer treatment.
    Language
    eng
    ISSN
    1534-4681 (Electronic)
    1068-9265 (Linking)
    ae974a485f413a2113503eed53cd6c53
    10.1245/s10434-008-0037-5
    Scopus Count
    Collections
    Cork University Hospital

    entitlement

    Related articles

    • Targeting fibroblast growth factor receptors blocks PI3K/AKT signaling, induces apoptosis, and impairs mammary tumor outgrowth and metastasis.
    • Authors: Dey JH, Bianchi F, Voshol J, Bonenfant D, Oakeley EJ, Hynes NE
    • Issue date: 2010 May 15
    • Identification of survival-related genes of the phosphatidylinositol 3'-kinase signaling pathway in glioblastoma multiforme.
    • Authors: Ruano Y, Mollejo M, Camacho FI, Rodríguez de Lope A, Fiaño C, Ribalta T, Martínez P, Hernández-Moneo JL, Meléndez B
    • Issue date: 2008 Apr 1
    • Loss of beta4 integrin subunit reduces the tumorigenicity of MCF7 mammary cells and causes apoptosis upon hormone deprivation.
    • Authors: Bon G, Folgiero V, Bossi G, Felicioni L, Marchetti A, Sacchi A, Falcioni R
    • Issue date: 2006 Jun 1
    • Gene expression profile by inhibiting Raf-1 protein kinase in breast cancer cells.
    • Authors: Mewani RR, Tian S, Li B, Danner MT, Carr TD, Lee S, Rahman A, Kasid UN, Jung M, Dritschilo A, Gokhale PC
    • Issue date: 2006 Mar
    • Increased NBS1 expression is a marker of aggressive head and neck cancer and overexpression of NBS1 contributes to transformation.
    • Authors: Yang MH, Chiang WC, Chou TY, Chang SY, Chen PM, Teng SC, Wu KJ
    • Issue date: 2006 Jan 15
    Health Library Ireland | Health Service Executive | Jervis House, Jervis Street | Republic of Ireland | Eircode: D01 W596
    lenus@hse.ie | Tel: +353-1-7786275
    DSpace software copyright © 2002-2017  DuraSpace
    Contact Us | Disclaimer
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.