The influence of propofol on P-selectin expression and nitric oxide production in re-oxygenated human umbilical vein endothelial cells.
Affiliation
Department of Anaesthesia, Cork University Hospital, Cork City, Republic of, Ireland. mascor@gofree.indigo.ieIssue Date
2012-02-03T15:05:44ZMeSH
Antioxidants/pharmacologyCell Hypoxia
Cells, Cultured
Endothelial Cells/*drug effects/metabolism
Humans
Nitric Oxide/*biosynthesis
Nitric Oxide Synthase Type III/analysis
Oxygen/*pharmacology
P-Selectin/*analysis
Propofol/*pharmacology
Reperfusion Injury/prevention & control
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Acta Anaesthesiol Scand. 2006 Mar;50(3):348-54.Journal
Acta anaesthesiologica ScandinavicaDOI
10.1111/j.1399-6576.2006.00955.xPubMed ID
16480469Abstract
BACKGROUND: Reperfusion injury is characterized by free radical production and endothelial inflammation. Neutrophils mediate much of the end-organ injury that occurs, requiring P-selectin-mediated neutrophil-endothelial adhesion, and this is associated with decreased endothelial nitric oxide production. Propofol has antioxidant properties in vitro which might abrogate this inflammation. METHODS: Cultured human umbilical vein endothelial cells were exposed to 20 h of hypoxia and then returned to normoxic conditions. Cells were treated with saline, Diprivan 5 microg/l or propofol 5 microg/l for 4 h after re-oxygenation and were then examined for P-selectin expression and supernatant nitric oxide concentrations for 24 h. P-selectin was determined by flow cytometry, and culture supernatant nitric oxide was measured as nitrite. RESULTS: In saline-treated cells, a biphasic increase in P-selectin expression was demonstrated at 30 min (P = 0.01) and 4 h (P = 0.023) after re-oxygenation. Propofol and Diprivan prevented these increases in P-selectin expression (P < 0.05). Four hours after re-oxygenation, propofol decreased endothelial nitric oxide production (P = 0.035). CONCLUSION: This is the first study to demonstrate an effect of propofol upon endothelial P-selectin expression. Such an effect may be important in situations of reperfusion injury such as cardiac transplantation and coronary artery bypass surgery. We conclude that propofol attenuates re-oxygenation-induced endothelial inflammation in vitro.Language
engISSN
0001-5172 (Print)0001-5172 (Linking)
ae974a485f413a2113503eed53cd6c53
10.1111/j.1399-6576.2006.00955.x
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