Bcl-x(L) expression in vivo in rheumatoid synovium.
dc.contributor.author | Busteed, S | |
dc.contributor.author | Bennett, M W | |
dc.contributor.author | Molloy, C | |
dc.contributor.author | Houston, A | |
dc.contributor.author | Stone, M A | |
dc.contributor.author | Shanahan, F | |
dc.contributor.author | Molloy, M G | |
dc.contributor.author | O'Connell, J | |
dc.date.accessioned | 2012-02-03T15:05:30Z | |
dc.date.available | 2012-02-03T15:05:30Z | |
dc.date.issued | 2012-02-03T15:05:30Z | |
dc.identifier.citation | Clin Rheumatol. 2006 Nov;25(6):789-93. Epub 2006 Mar 30. | en_GB |
dc.identifier.issn | 0770-3198 (Print) | en_GB |
dc.identifier.issn | 0770-3198 (Linking) | en_GB |
dc.identifier.pmid | 16572289 | en_GB |
dc.identifier.doi | 10.1007/s10067-005-0191-0 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10147/208853 | |
dc.description.abstract | To examine the expression of the apoptosis regulatory protein, Bcl-x(L), in the synovium of patients with rheumatoid arthritis (RA) and osteoarthritis (OA). Immunohistochemistry for Bcl-x(L) was carried out on synovial samples from patients with RA and OA. Reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot analysis were performed to qualitatively examine the expression of Bcl-x(L). Bcl-x(L) expression was detected in the lining, endothelium and inflammatory cells of both RA (n=20) and OA (n=10) samples. However, there was significantly more expression in the lining of RA synovium compared to OA (77 vs 61%, p<0.05). Many of the positive cells in the RA subsynovium were noted to be plasma cells. There was a significant correlation between Bcl-x(L) expression and the number of inflammatory cells in the subsynovium of RA and OA patients (r (s)=0.376, p<0.05, n=30). Age and disease duration did not correlate with Bcl-x(L) expression in rheumatoid patients. Bcl-x(L) may play a role in the extended survival of synoviocytes and inflammatory cells in rheumatoid synovium. | |
dc.language.iso | eng | en_GB |
dc.subject.mesh | Aged | en_GB |
dc.subject.mesh | Aging/metabolism | en_GB |
dc.subject.mesh | Arthritis, Rheumatoid/*metabolism/pathology | en_GB |
dc.subject.mesh | Blotting, Western | en_GB |
dc.subject.mesh | Endothelial Cells/metabolism | en_GB |
dc.subject.mesh | Female | en_GB |
dc.subject.mesh | Humans | en_GB |
dc.subject.mesh | Immunohistochemistry | en_GB |
dc.subject.mesh | Male | en_GB |
dc.subject.mesh | Middle Aged | en_GB |
dc.subject.mesh | Osteoarthritis/metabolism/pathology | en_GB |
dc.subject.mesh | Plasma Cells/metabolism | en_GB |
dc.subject.mesh | RNA, Messenger/metabolism | en_GB |
dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | en_GB |
dc.subject.mesh | Staining and Labeling | en_GB |
dc.subject.mesh | Synovial Membrane/*metabolism/pathology | en_GB |
dc.subject.mesh | Time Factors | en_GB |
dc.subject.mesh | bcl-X Protein/genetics/*metabolism | en_GB |
dc.title | Bcl-x(L) expression in vivo in rheumatoid synovium. | en_GB |
dc.contributor.department | Department of Medicine, Cork University Hospital, National University of Ireland,, Wilton, Cork, Ireland. sandra_busteed@hotmail.com | en_GB |
dc.identifier.journal | Clinical rheumatology | en_GB |
dc.description.province | Munster | |
html.description.abstract | To examine the expression of the apoptosis regulatory protein, Bcl-x(L), in the synovium of patients with rheumatoid arthritis (RA) and osteoarthritis (OA). Immunohistochemistry for Bcl-x(L) was carried out on synovial samples from patients with RA and OA. Reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot analysis were performed to qualitatively examine the expression of Bcl-x(L). Bcl-x(L) expression was detected in the lining, endothelium and inflammatory cells of both RA (n=20) and OA (n=10) samples. However, there was significantly more expression in the lining of RA synovium compared to OA (77 vs 61%, p<0.05). Many of the positive cells in the RA subsynovium were noted to be plasma cells. There was a significant correlation between Bcl-x(L) expression and the number of inflammatory cells in the subsynovium of RA and OA patients (r (s)=0.376, p<0.05, n=30). Age and disease duration did not correlate with Bcl-x(L) expression in rheumatoid patients. Bcl-x(L) may play a role in the extended survival of synoviocytes and inflammatory cells in rheumatoid synovium. |