The influence of propofol on the expression of intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) in reoxygenated human umbilical vein endothelial cells.
Affiliation
Cork University Hospital, Department of Anaesthesia, Cork City, Republic of, IrelandUniversity College Cork, Department of Anaesthesia, Cork City, Republic of, Ireland. mascor@gofree.indigo.ieIssue Date
2012-02-03T15:04:51ZMeSH
Analysis of VarianceAnesthetics, Intravenous/*therapeutic use
Cell Hypoxia/physiology
Cells, Cultured
Endothelial Cells/*drug effects/metabolism
Endothelium, Vascular/cytology
Humans
Intercellular Adhesion Molecule-1/*metabolism
Leukocytes/immunology
Oxygen/metabolism
Propofol/*therapeutic use
Time Factors
Umbilical Veins
Vascular Cell Adhesion Molecule-1/*metabolism
Metadata
Show full item recordCitation
Eur J Anaesthesiol. 2006 Nov;23(11):942-7. Epub 2006 Jul 11.Journal
European journal of anaesthesiologyDOI
10.1017/S0265021506000846PubMed ID
16834788Abstract
BACKGROUND: Leucocytes are a pivotal component of the inflammatory cascade that results in tissue injury in a large group of disorders. Free radical production and endothelial activation promote leucocyte-endothelium interactions via endothelial expression of vascular cell adhesion molecule 1 (VCAM-1) and intercellular adhesion molecule 1 (ICAM-1) which augment these processes, particularly in the setting of reperfusion injury. Propofol has antioxidant properties which may attenuate the increased expression of these molecules that is observed. METHODS: Cultured human umbilical vein endothelial cells were exposed to 20 h of hypoxia, then returned to normoxic conditions. Cells were treated with saline, Diprivan 5 microg mL(-1) or propofol 5 microg mL(-1), for 4 h after reoxygenation and were examined for ICAM-1 and VCAM-1 expression. RESULTS: Hypoxia did not increase the expression of ICAM-1/VCAM-1. ICAM-1 expression peaked 12 h after reoxygenation (21.75(0.6) vs. 9.6(1.3), P = 0.02). Propofol, but not Diprivan, prevented this increase (8.2(2.9) vs. 21.75(0.6), P = 0.009). VCAM-1 expression peaked 24 h after reoxygenation (9.8(0.9) vs. 6.6(0.6), P = 0.03). Propofol and Diprivan prevented this increase, with no difference between the two treatments observed (4.3(0.3) and 6.4(0.5) vs. 9.8(0.9), P = 0.001, 0.02, respectively). CONCLUSION: These effects are likely to be attributable to the antioxidant properties of propofol, and suggest that propofol may have a protective role in disorders where free radical mediated injury promotes leucocyte-endothelium adhesive interactions.Language
engISSN
0265-0215 (Print)0265-0215 (Linking)
ae974a485f413a2113503eed53cd6c53
10.1017/S0265021506000846
Scopus Count
Collections
Related articles
- The influence of propofol on P-selectin expression and nitric oxide production in re-oxygenated human umbilical vein endothelial cells.
- Authors: Corcoran TB, O'Shea A, Engel A, Shorten GD
- Issue date: 2006 Mar
- Short-chain fatty acids modulate gene expression for vascular endothelial cell adhesion molecules.
- Authors: Miller SJ, Zaloga GP, Hoggatt AM, Labarrere C, Faulk WP
- Issue date: 2005 Jun
- Adenovirus viral interleukin-10 inhibits adhesion molecule expressions induced by hypoxia/reoxygenation in cerebrovascular endothelial cells.
- Authors: Kang H, Yang PY, Rui YC
- Issue date: 2008 Jan
- The effect of midazolam on cerebral endothelial (P-selectin and ICAM-1) adhesion molecule expression during hypoxia-reperfusion injury in vitro.
- Authors: Ghori K, Harmon D, Lan W, Seigne P, Walsh F, Shorten GD
- Issue date: 2008 Mar
- [Inhibitory effects of ligustrazine on the expression of intercellular cell adhesion molecule-1, vascular cell adhesion molecule-1 in human umbilical vein endothelial cells induced by preeclamptic plasma].
- Authors: Zeng S, Fan S, Jiang X
- Issue date: 2000 May