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dc.contributor.authorLaing, A J
dc.contributor.authorDillon, J P
dc.contributor.authorCondon, E T
dc.contributor.authorStreet, J T
dc.contributor.authorWang, J H
dc.contributor.authorMcGuinness, A J
dc.contributor.authorRedmond, H P
dc.date.accessioned2012-02-03T15:04:23Z
dc.date.available2012-02-03T15:04:23Z
dc.date.issued2012-02-03T15:04:23Z
dc.identifier.citationJ Orthop Res. 2007 Jan;25(1):44-50.en_GB
dc.identifier.issn0736-0266 (Print)en_GB
dc.identifier.issn0736-0266 (Linking)en_GB
dc.identifier.pmid17001704en_GB
dc.identifier.doi10.1002/jor.20228en_GB
dc.identifier.urihttp://hdl.handle.net/10147/208819
dc.description.abstractPostnatal vasculogenesis, the process by which vascular committed bone marrow stem cells or endothelial precursor cells (EPC) migrate, differentiate, and incorporate into the nacent endothelium contributing to physiological and pathological neovascularization, has stimulated much interest. Its contribution to tumor nonvascularization, wound healing, and revascularization associated with skeletal and cardiac muscles ischaemia is established. We evaluated the mobilization of EPCs in response to musculoskeletal trauma. Blood from patients (n = 15) following AO type 42a1 closed diaphyseal tibial fractures was analyzed for CD34 and AC133 cell surface marker expression. Immunomagnetically enriched CD34+ mononuclear cell (MNC(CD34+)) populations were cultured and examined for phenotypic and functional vascular endothelial differentiation. Circulating MNC(CD34+) levels increased sevenfold by day 3 postinjury. Circulating MNC(AC133+) increased 2.5-fold. Enriched MNC(CD34+) populations from day 3 samples in culture exhibited cell cluster formation with sprouting spindles. These cells bound UEA-1 and incorporated fluorescent DiI-Ac-LDL intracellularily. Our findings suggest a systemic provascular response is initiated in response to musculoskeletal trauma. Its therapeutic manipulation may have implications for the potential enhancement of fracture healing.
dc.language.isoengen_GB
dc.subject.meshAdulten_GB
dc.subject.meshAntigens, CD34/physiologyen_GB
dc.subject.meshCell Differentiationen_GB
dc.subject.meshEndothelial Cells/cytologyen_GB
dc.subject.meshEndothelium, Vascular/*cytologyen_GB
dc.subject.meshFemaleen_GB
dc.subject.meshHumansen_GB
dc.subject.meshLeukocytes, Mononuclear/immunology/physiologyen_GB
dc.subject.meshMaleen_GB
dc.subject.meshMiddle Ageden_GB
dc.subject.meshNeovascularization, Physiologic/*physiologyen_GB
dc.subject.meshStem Cells/*cytologyen_GB
dc.subject.meshTibial Fractures/*physiopathologyen_GB
dc.titleMobilization of endothelial precursor cells: systemic vascular response to musculoskeletal trauma.en_GB
dc.contributor.departmentDepartments of Surgical Research and Orthopaedic Surgery, Cork University, Hospital, Cork, Ireland. alanjlaing@hotmail.comen_GB
dc.identifier.journalJournal of orthopaedic research : official publication of the Orthopaedic, Research Societyen_GB
dc.description.provinceMunster
html.description.abstractPostnatal vasculogenesis, the process by which vascular committed bone marrow stem cells or endothelial precursor cells (EPC) migrate, differentiate, and incorporate into the nacent endothelium contributing to physiological and pathological neovascularization, has stimulated much interest. Its contribution to tumor nonvascularization, wound healing, and revascularization associated with skeletal and cardiac muscles ischaemia is established. We evaluated the mobilization of EPCs in response to musculoskeletal trauma. Blood from patients (n = 15) following AO type 42a1 closed diaphyseal tibial fractures was analyzed for CD34 and AC133 cell surface marker expression. Immunomagnetically enriched CD34+ mononuclear cell (MNC(CD34+)) populations were cultured and examined for phenotypic and functional vascular endothelial differentiation. Circulating MNC(CD34+) levels increased sevenfold by day 3 postinjury. Circulating MNC(AC133+) increased 2.5-fold. Enriched MNC(CD34+) populations from day 3 samples in culture exhibited cell cluster formation with sprouting spindles. These cells bound UEA-1 and incorporated fluorescent DiI-Ac-LDL intracellularily. Our findings suggest a systemic provascular response is initiated in response to musculoskeletal trauma. Its therapeutic manipulation may have implications for the potential enhancement of fracture healing.


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