Mobilization of endothelial precursor cells: systemic vascular response to musculoskeletal trauma.
Affiliation
Departments of Surgical Research and Orthopaedic Surgery, Cork University, Hospital, Cork, Ireland. alanjlaing@hotmail.comIssue Date
2012-02-03T15:04:23ZMeSH
AdultAntigens, CD34/physiology
Cell Differentiation
Endothelial Cells/cytology
Endothelium, Vascular/*cytology
Female
Humans
Leukocytes, Mononuclear/immunology/physiology
Male
Middle Aged
Neovascularization, Physiologic/*physiology
Stem Cells/*cytology
Tibial Fractures/*physiopathology
Metadata
Show full item recordCitation
J Orthop Res. 2007 Jan;25(1):44-50.Journal
Journal of orthopaedic research : official publication of the Orthopaedic, Research SocietyDOI
10.1002/jor.20228PubMed ID
17001704Abstract
Postnatal vasculogenesis, the process by which vascular committed bone marrow stem cells or endothelial precursor cells (EPC) migrate, differentiate, and incorporate into the nacent endothelium contributing to physiological and pathological neovascularization, has stimulated much interest. Its contribution to tumor nonvascularization, wound healing, and revascularization associated with skeletal and cardiac muscles ischaemia is established. We evaluated the mobilization of EPCs in response to musculoskeletal trauma. Blood from patients (n = 15) following AO type 42a1 closed diaphyseal tibial fractures was analyzed for CD34 and AC133 cell surface marker expression. Immunomagnetically enriched CD34+ mononuclear cell (MNC(CD34+)) populations were cultured and examined for phenotypic and functional vascular endothelial differentiation. Circulating MNC(CD34+) levels increased sevenfold by day 3 postinjury. Circulating MNC(AC133+) increased 2.5-fold. Enriched MNC(CD34+) populations from day 3 samples in culture exhibited cell cluster formation with sprouting spindles. These cells bound UEA-1 and incorporated fluorescent DiI-Ac-LDL intracellularily. Our findings suggest a systemic provascular response is initiated in response to musculoskeletal trauma. Its therapeutic manipulation may have implications for the potential enhancement of fracture healing.Language
engISSN
0736-0266 (Print)0736-0266 (Linking)
ae974a485f413a2113503eed53cd6c53
10.1002/jor.20228